{"product_id":"proteintech-12601-1-ap","title":"Proteintech, 12601-1-AP, GCLC Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe GCLC (12601-1-AP) by Proteintech is a Polyclonal antibody targeting GCLC in WB, IHC, IP, ELISA applications with reactivity to human, mouse, rat samples\n12601-1-AP targets GCLC in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: Jurkat cells, Daudi cells,  HEK-293 cells,  L02 cells,  mouse kidney tissue,  mouse lung tissue,  Raji cells,  rat lung tissue\nPositive IP detected in: mouse kidney tissue\nPositive IHC detected in: human kidney tissue, human liver tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:1000-1:4000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nGCLC (Glutamate--cysteine ligase catalytic subunit) belongs to the glutamate--cysteine ligase type 3 family.It is also named as GLCL, GLCLC.It is the first rate-limiting enzyme in glutathione (GSH) biosynthesis.GCLC) gene may be associated with coronary endothelial vasomotor dysfunction and myocardial infarction (MI) (PMID:12598062). Defects in GCLC are the cause of hemolytic anemia (HAGGSD).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, mouse, rat, goat\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag3252 Product name: Recombinant human GCLC protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-252 aa of BC022487 Sequence: MGLLSQGSPLSWEETKRHADHVRRHGILQFLHIYHAVKDRHKDVLKWGDEVEYMLVSFDHENKKVRLVLSGEKVLETLQEKGERTNPNHPTLWRPEYGSYMIEGTPGQPYGGTMSEFNTVEANMRKRRKEATSILEENQALCTITSFPRLGCPGFTLPEVKPNPVEGGASKSLFFPDEAINKHPRFSTLTRNIRHRRGEKVVINVPIFKDKNTPSPFIETFTEDDEASRASKPDHIYMDAMGFGMGNCCLQV Predict reactive species\nFull Name: glutamate-cysteine ligase, catalytic subunit\nCalculated Molecular Weight: 637 aa, 73 kDa\nObserved Molecular Weight: 73 kDa\nGenBank Accession Number: BC022487\nGene Symbol: GCLC\nGene ID (NCBI): 2729\nRRID: AB_2278734\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: P48506\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868825440425,"sku":"12601-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-12601-1-ap","provider":"Iright","version":"1.0","type":"link"}