{"product_id":"proteintech-12807-1-ap","title":"Proteintech, 12807-1-AP, BACE1 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe BACE1 (12807-1-AP) by Proteintech is a Polyclonal antibody targeting BACE1 in WB, IHC, IF-P, IF-Fro, IP, ELISA applications with reactivity to human, mouse, rat, zebrafish samples\n12807-1-AP targets BACE1 in WB, IHC, IF-P, IF-Fro, IP, ELISA applications and shows reactivity with human, mouse, rat, zebrafish samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: mouse brain tissue, human brain tissue,  mouse pancreas tissue,  HeLa cells,  zebrafish tissue,  PC-12 cells,  rat brain tissue\nPositive IP detected in: mouse brain tissue\nPositive IHC detected in: mouse brain tissue,  human pancreas cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF-P detected in: mouse brain tissue\nPositive IF-Fro detected in: mouse brain tissue\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:1000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:100-1:400\nImmunofluorescence (IF)-P: IF-P : 1:50-1:500\nImmunofluorescence (IF)-FRO: IF-FRO : 1:200-1:800\n\u003cb\u003eBackground Information\u003c\/b\u003e\nbeta-secretase, encoded by BACE1(beta-stie amyloid beta A4 precursor protein-cleavage enzyem1) gene, is a neuron-specific membrane-associated protease that participate in the two step proteolytic processing of the APP. Cleaves at the N-terminus of the A-beta peptide sequence, between residues 671 and 672 of APP, leads to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase. It has a high expression in the brain and pancreas. This protein has 6 isoforms produced by alternative splicing. BACE1 can be detected with different molecular weight: 42-56kD (prozymogen and isoforms), 65-75 (glycosylated forms) and 160kDa (dimer) (PMID:22741101; 21795680).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat, zebrafish\nCited Reactivity: human, mouse, rat\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag3559 Product name: Recombinant human BACE1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 157-457 aa of BC036084 Sequence: VRANIAAITESDKFFINGSNWEGILGLAYAEIARPDDSLEPFFDSLVKQTHVPNLFSLQLCGAGFPLNQSEVLASVGGSMIIGGIDHSLYTGSLWYTPIRREWYYEVIIVRVEINGQDLKMDCKEYNYDKSIVDSGTTNLRLPKKVFEAAVKSIKAASSTEKFPDGFWLGEQLVCWQAGTTPWNIFPVISLYLMGEVTNQSFRITILPQQYLRPVEDVATSQDDCYKFAISQSSTGTVMGAVIMEGFYVVFDRARKRIGFAVSACHVHDEFRTAAVEGPFVTLDMEDCGYNIPQTDESTLM Predict reactive species\nFull Name: beta-site APP-cleaving enzyme 1\nCalculated Molecular Weight: 501 aa, 56 kDa\nObserved Molecular Weight: 70-75 kDa, 42-56 kDa\nGenBank Accession Number: BC036084\nGene Symbol: BACE1\nGene ID (NCBI): 23621\nRRID: AB_10638760\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: P56817\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868851851433,"sku":"12807-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-12807-1-ap","provider":"Iright","version":"1.0","type":"link"}