{"product_id":"proteintech-12825-1-ap","title":"Proteintech, 12825-1-AP, MLLT3\/AF9 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe MLLT3\/AF9 (12825-1-AP) by Proteintech is a Polyclonal antibody targeting MLLT3\/AF9 in WB, ELISA applications with reactivity to human, mouse, rat samples\n12825-1-AP targets MLLT3\/AF9 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: Jurkat cells, HL-60 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:1000\n\u003cb\u003eBackground Information\u003c\/b\u003e\nMLLT3, also named as AF9, YEATS3, is a 568 amino acid protein, which is a component of the superelongation complex6 and co-operates with DOT1L, which di\/trimethylates H3K79 to promote transcription. MLLT3 localizes to active transcription start sites (TSSs) through the YEATS domain, which recognizes active histone marks such as H3K9 acetylation and crotonylation. A truncated MLLT3 that lacks the YEATS domain forms a leukaemic fusion protein with the N terminus of MLL1, which misdirects MLLT3-interacting complexes to induce aberrant gene transcription. MLLT3 also regulates erythroid or megakaryocytic progenitors and was identified as a definitive HSC hub gene during mouse development. Researcher found that the expression of a gene called MLLT3 was closely correlated with blood stem cells'potential to self-renew and that the protein generated by the MLLT3 gene provides blood stem cells with the instructions necessary to maintain its ability to self-renew. It does this by working with other regulatory proteins to keep important parts of the blood stem cell's machinery operational as the cells divide.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag3528 Product name: Recombinant human MLLT3 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-348 aa of BC036089 Sequence: MASSCAVQVKLELGHRAQVRKKPTVEGFTHDWMVFVRGPEHSNIQHFVEKVVFHLHESFPRPKRVCKDPPYKVEESGYAGFILPIEVYFKNKEEPRKVRFDYDLFLHLEGHPPVNHLRCEKLTFNNPTEDFRRKLLKAGGDPNRSIHTSSSSSSSSSSSSSSSSSSSSSSSSGSSSSSSSSSSSSSSSSSTSFSKPHKLMKEHKEKPSKDSREHKSAFKEPSRDHNKSSKESSKKPKENKPLKEEKIVPKMAFKEPKPMSKEPKPDSNLLTITSGQDKKAPSKRPPISDSEELSAKKRKKSSSEALFKSFSSAPPLILTCSADKKQIKDKSHVKMGKVKIESETSEKK Predict reactive species\nFull Name: myeloid\/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to, 3\nCalculated Molecular Weight: 568 aa, 63 kDa\nObserved Molecular Weight: 63-70 kDa\nGenBank Accession Number: BC036089\nGene Symbol: AF9\/MLLT3\nGene ID (NCBI): 4300\nRRID: AB_2145949\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: P42568\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46887720616105,"sku":"12825-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-12825-1-ap","provider":"Iright","version":"1.0","type":"link"}