{"product_id":"proteintech-13361-1-ap","title":"Proteintech, 13361-1-AP, PIP5K3 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe PIP5K3 (13361-1-AP) by Proteintech is a Polyclonal antibody targeting PIP5K3 in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples\n13361-1-AP targets PIP5K3 in WB, IHC, Dot Blot, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HeLa cells, mouse lung tissue,  Jurkat cells,  mouse brain tissue,  rat brain tissue\nPositive IHC detected in: human liver cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:1000-1:4000\nImmunohistochemistry (IHC): IHC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nPIP5K3 (1-phosphatidylinositol 3-phosphate 5-kinase), also callled PIKFYVE, belongs to a large family of lipid kinases that alter the phosphorylation status of intracellular phosphatidylinositol (PMID: 15902656; 9858586). The content of phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2) in endosomal membranes changes dynamically with fission and fusion events that generate or absorb intracellular transport vesicles. PIKFYVE is the PtdIns(3,5)P2-producing component of a trimolecular complex that tightly regulates the level of PtdIns(3,5)P2. Other components of this complex are the PIKFYVE activator VAC14 and the PtdIns(3,5)P2 phosphatase FIG4 (PMID: 17556371). In addition to its phosphoinositide 5-kinase activity, PIKFYVE also has protein kinase activity (PubMed: 11714711; 17556371).PIP5K3 has four isoforms with molecular weights of 237 kDa, 50 kDa, 51 kDa and 62 kDa. This antibody primarily recognizes the 237 kDa isoform.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, mouse, pig, sheep\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag4177 Product name: Recombinant human PIP5K3 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-451 aa of BC032389 Sequence: MATDDKTSPTLDSANDLPRSPTSPSHLTHFKPLTPDQDEPPFKSAYSSFVNLFRFNKERAEGGQGEQQPLSGSWTSPQLPSRTQSVRSPTPYKKQLNEELQRRSSALGDLRACTYCRKIALSYAHSTDSNSIGEDLNALSDSACSVSVLDPSEPRTPVGSRKASRNIFLEDDLAWQSLIHPDSSNTPLSTRLVSVQEDAGKSPARNRSASITNLSLDRSGSPMVPSYETSVSPQANRTYVRTETTEDERKILLDSVQLKDLWKKICHHSSGMEFQDHRYWLRTHPNCIVGKELVNWLIRNGHIATRAQAIAIGQAMVDGRWLDCVSHHDQLFRDEYALYRPLQSTEFSETPSPDSDSVNSVEGHSEPSWFKDIKFDDSDTEQIAEEGDDNLANSASPSKRTSVSSFQSTVDSDSAASISLNVELDNVNFHIKKPSKYPHVPPHPADQKGRR Predict reactive species\nFull Name: phosphatidylinositol-3-phosphate\/phosphatidylinositol 5-kinase, type III\nCalculated Molecular Weight: 451 aa, 50 kDa, 237 kDa\nObserved Molecular Weight: 237 kDa\nGenBank Accession Number: BC032389\nGene Symbol: PIP5K3\nGene ID (NCBI): 200576\nRRID: AB_10638310\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q9Y2I7\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868935868585,"sku":"13361-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-13361-1-ap","provider":"Iright","version":"1.0","type":"link"}