{"product_id":"proteintech-13389-1-ap","title":"Proteintech, 13389-1-AP, PFKP Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe PFKP (13389-1-AP) by Proteintech is a Polyclonal antibody targeting PFKP in WB, IHC, IF\/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples\n13389-1-AP targets PFKP in WB, IHC, IF\/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HEK-293 cells, BxPC-3 cells,  HeLa cells,  human heart tissue,  Jurkat cells,  MCF-7 cells,  Raji cells,  mouse heart tissue,  mouse skeletal muscle tissue,  rat heart tissue,  rat skeletal muscle tissue,  NIH\/3T3 cells\nPositive IP detected in: Jurkat cells, mouse heart tissue\nPositive IHC detected in: mouse heart tissue, human lung cancer tissue,  human heart tissue,  human spleen tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: HeLa cells, BxPC-3 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:2000-1:16000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:50-1:500\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nPFKP(6-phosphofructokinase, platelet type) is also named as PFKF, PFK-C and belongs to the phosphofructokinase family. This form of PFK is best called the 'platelet' type and symbolized PFKP because it is the only form made by platelets, whereas fibroblasts have more than one form of PFK (Francke (1983)). PFK catalyzes the irreversible conversion of fructose-6-phosphate to fructose-1,6-bisphosphate and is a key regulatory enzyme in glycolysis. The observed molecular weight of PFKP monomer is 80-86 kDa. The molecular mass of PFKP dimer is 171 kDa (PMID: 28607489).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, mouse, rat\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag4047 Product name: Recombinant human PFKP protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 436-784 aa of BC029138 Sequence: GIADGHRMLAIYDGFDGFAKGQIKEIGWTDVGGWTGQGGSILGTKRVLPGKYLEEIATQMRTHSINALLIIGGFEAYLGLLELSAAREKHEEFCVPMVMVPATVSNNVPGSDFSIGADTALNTITDTCDRIKQSASGTKRRVFIIETMGGYCGYLANMGGLAAGADAAYIFEEPFDIRDLQSNVEHLTEKMKTTIQRGLVLRNESCSENYTTDFIYQLYSEEGKGVFDCRKNVLGHMQQGGAPSPFDRNFGTKISARAMEWITAKLKEARGRGKKFTTDDSICVLGISKRNVIFQPVAELKKQTDFEHRIPKEQWWLKLRPLMKILAKYKASYDVSDSGQLEHVQPWSV Predict reactive species\nFull Name: phosphofructokinase, platelet\nCalculated Molecular Weight: 784 aa, 86 kDa\nObserved Molecular Weight: 80-86 kDa\nGenBank Accession Number: BC029138\nGene Symbol: PFKP\nGene ID (NCBI): 5214\nRRID: AB_2252278\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q01813\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868852211881,"sku":"13389-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-13389-1-ap","provider":"Iright","version":"1.0","type":"link"}