{"product_id":"proteintech-13398-1-ap","title":"Proteintech, 13398-1-AP, GPR161 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe GPR161 (13398-1-AP) by Proteintech is a Polyclonal antibody targeting GPR161 in WB, IF\/ICC, ELISA applications with reactivity to human, mouse, canine samples\n13398-1-AP targets GPR161 in WB, IHC, IF\/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, canine samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: mouse brain tissue\nPositive IF\/ICC detected in: MDCK cells, ARPE-19 cells,  C2C12 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:1000-1:8000\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:200-1:800\n\u003cb\u003eBackground Information\u003c\/b\u003e\nGPR161 (also known as RE2) is an orphan G protein-coupled receptor and plays an important role in the Hh pathway. In the absence of Hh signals, GPR161 localizes to primary cilia and keeps the downstream GLI transcription factors in their repressor forms (PMID: 26305592). Ciliary localization of GPR161 requires TULP3 and the IFT-A complex. In the presence of SHH, GPR161 is removed from primary cilia and is internalized into recycling endosomes, preventing its activity and allowing activation of the Shh signaling. GPR161 has a calculated molecular mass of 59 kDa. The 70-kDa band probably represents a modified version of GPR161 (PMID: 18250320).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, canine\nCited Reactivity: human, mouse, pig, chicken, zebrafish, bovine\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag4221 Product name: Recombinant human GPR161 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 321-529 aa of BC028163 Sequence: PLIYGLWNKTVRKELLGMCFGDRYYREPFVQRQRTSRLFSISNRITDLGLSPHLTALMAGGQPLGHSSSTGDTGFSCSQDSGTDMMLLEDYTSDDNPPSHCTCPPKRRSSVTFEDEVEQIKEAAKNSILHVKAEVHKSLDSYAASLAKAIEAEAKINLFGEEALPGVLVTARTVPGGGFGGRRGSRTLVSQRLQLQSIEEGDVLAAEQR Predict reactive species\nFull Name: G protein-coupled receptor 161\nCalculated Molecular Weight: 529 aa, 59 kDa\nObserved Molecular Weight: 59 kDa\nGenBank Accession Number: BC028163\nGene Symbol: GPR161\nGene ID (NCBI): 23432\nRRID: AB_2113965\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q8N6U8\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868849623209,"sku":"13398-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-13398-1-ap","provider":"Iright","version":"1.0","type":"link"}