{"product_id":"proteintech-13511-1-ap","title":"Proteintech, 13511-1-AP, Beta-2-Microglobulin Polyclonal antibody","description":"Size: 20ul \/ 150ul\nBeta-2-Microglobulin Polyclonal Antibody for WB, IHC, IF\/ICC, IP, ELISA\n13511-1-AP targets Beta-2-Microglobulin in WB, IHC, IF\/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: A431 cells, Hepa1-6 cells,  human heart tissue,  human stomach tissue,  mouse lung tissue,  HeLa cells,  HepG2 cells,  Jurkat cells,  Raji cells,  mouse spleen tissue,  rat spleen tissue,  rat lung tissue\nPositive IP detected in: A431 cells\nPositive IHC detected in: human tonsillitis tissue, mouse lung tissue,  human liver cancer tissue,  human prostate cancer tissue,  human oesophagus cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: A431 cells, NCCIT cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:2000-1:16000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:2000-1:8000\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:375-1:1500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nBeta-2-microglobulin (B2M) is a component of MHC class I molecules, which are present on the surface of nearly all nucleated cells. It can be found in body fluids under physiologic conditions as a result of shedding from cell surfaces or intracellular release. B2M has various biological functions, including antigen presentation. Investigations reveal that increased synthesis and release of B2M are present in several malignant diseases.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, mouse, rat\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag4433 Product name: Recombinant human B2M protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 22-119 aa of BC032589 Sequence: QRTPKIQVYSRHPAENGKSNFLNCYVSGFHPSDIEVDLLKNGERIEKVEHSDLSFSKDWSFYLLYYTEFTPTEKDEYACRVNHVTLSQPKIVKWDRDM Predict reactive species\nFull Name: beta-2-microglobulin\nCalculated Molecular Weight: 119 aa, 14 kDa\nObserved Molecular Weight: 12-14 kDa\nGenBank Accession Number: BC032589\nGene Symbol: B2M\nGene ID (NCBI): 567\nENSEMBL Gene ID: ENSG00000166710\nRRID: AB_2062735\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: P61769\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868862533801,"sku":"13511-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-13511-1-ap","provider":"Iright","version":"1.0","type":"link"}