{"product_id":"proteintech-13614-1-ap","title":"Proteintech, 13614-1-AP, MLXIP Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe MLXIP (13614-1-AP) by Proteintech is a Polyclonal antibody targeting MLXIP in WB, IHC, IP, ELISA applications with reactivity to human samples\n13614-1-AP targets MLXIP in WB, IHC, IF, IP, ChIP, ELISA applications and shows reactivity with human samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: K-562 cells\nPositive IP detected in: K-562 cells\nPositive IHC detected in: human skeletal muscle tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:200-1:1000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:20-1:200\n\u003cb\u003eBackground Information\u003c\/b\u003e\nMlx-interacting protein(MLXIP), after forming heterodimers with MLX, can bind to and then activate transcription from CACGTG E boxes. The N terminus of MLXIP acts as a CRM1-dependent nuclear export signal and contribute to the cytoplasmic localization of the complex, and as a binding site for 13 protein. the C terminus contains a cytoplasmic localization signal and mediates protein-protein interaction of MONDOA and MLX. The complex play a role in transcriptional activation of glycolytic target genes and glucose-responsive gene regulation. This antibody could recognize isoform1(~110kd) and isoform3(~69kd) of MLXIP. A prominent and specific MLXIP of approximately 130 kDa was detected. It demonstrates that MLXIP associated specifically with Mlx  in the cytoplasm (PMID: 11073985).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human\nCited Reactivity: human, mouse, rat\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag4519 Product name: Recombinant human MLXIP protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-217 aa of BC039704 Sequence: MDGQGCEHTSRTEDPFIQPTDFGPSEPPLSVPPPISPVLPLVPPPATALNPPAPPTFHQPQKFAGVNKAPSVITHTASATLTHDAPATTFSQSQGLVITTHHPAPSAAPCGLALSPVTRPPQPRLTFVHPKPVSLTGGRPKQPHKIVPAPKPEPVSLVLKNARIAPGEPGGETQCGAPPDPEGCFPIPKAFKLVTTTTTLVCTCMRTHIHLNETKVS Predict reactive species\nFull Name: MLX interacting protein\nCalculated Molecular Weight: 919 aa, 100 kDa\nObserved Molecular Weight: 130 kDa, 69 kDa\nGenBank Accession Number: BC039704\nGene Symbol: MLXIP\nGene ID (NCBI): 22877\nRRID: AB_2282153\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q9HAP2\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868874100905,"sku":"13614-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-13614-1-ap","provider":"Iright","version":"1.0","type":"link"}