{"product_id":"proteintech-13618-1-ap","title":"Proteintech, 13618-1-AP, TTL Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe TTL (13618-1-AP) by Proteintech is a Polyclonal antibody targeting TTL in WB, IHC, IF-P, ELISA applications with reactivity to human, mouse, rat samples\n13618-1-AP targets TTL in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HeLa cells, mouse brain,  rat brain\nPositive IHC detected in: mouse brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF-P detected in: mouse brain tissue\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:1000-1:4000\nImmunohistochemistry (IHC): IHC : 1:50-1:500\nImmunofluorescence (IF)-P: IF-P : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nTubulin-tyrosine ligase (TTL) is the enzyme responsible for the reversible addition of a tyrosine residue at the carboxyl end of alpha-tubulin. TTL forms stable complexes with tubulin and inhibit tubulin polymerization. TTL is frequently suppressed during tumor progression with resulting accumulation of detyrosinated alpha-tubulin in tumor cells. TTL suppression in human cancers is associated with increased tumor aggressiveness.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, mouse, rat, canine\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag4526 Product name: Recombinant human TTL protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-377 aa of BC036819 Sequence: MYTFVVRDENSSVYAEVSRLLLATGHWKRLRRDNPRFNLMLGERNRLPFGRLGHEPGLVQLVNYYRGADKLCRKASLVKLIKTSPELAESCTWFPESYVIYPTNLKTPVAPAQNGIQPPISNSRTDEREFFLASYNRKKEDGEGNVWIAKSSAGAKGEGILISSEASELLDFIDNQGQVHVIQKYLEHPLLLEPGHRKFDIRSWVLVDHQYNIYLYREGVLRTASEPYHVDNFQDKTCHLTNHCIQKEYSKNYGKYEEGNEMFFKEFNQYLTSALNITLESSILLQIKHIIRNCLLSVEPAISTKHLPYQSFQLFGFDFMVDEELKVWLIEVNGAPACAQKLYAELCQGIVDIAISSVFPPPDVEQPQTQPAAFIKL Predict reactive species\nFull Name: tubulin tyrosine ligase\nCalculated Molecular Weight: 377 aa, 43 kDa\nObserved Molecular Weight: 43-45 kDa\nGenBank Accession Number: BC036819\nGene Symbol: TTL\nGene ID (NCBI): 150465\nRRID: AB_2256858\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q8NG68\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868856111273,"sku":"13618-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-13618-1-ap","provider":"Iright","version":"1.0","type":"link"}