{"product_id":"proteintech-13766-1-ap","title":"Proteintech, 13766-1-AP, Entactin Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe Entactin (13766-1-AP) by Proteintech is a Polyclonal antibody targeting Entactin in WB, IHC, IF\/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples\n13766-1-AP targets Entactin in WB, IHC, IF\/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: human placenta tissue, mouse heart tissue,  mouse placenta tissue\nPositive IP detected in: human placenta tissue\nPositive IHC detected in: human stomach cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: HepG2 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:1000-1:4000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:200-1:800\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nNidogen 1 (Entactin), first identified from a basement membrane-secreting cell line and the murine EHS tumor, is together with perlecan, laminin, and collagen IV, a ubiquitous component of basement membranes (PMID: 19530222). Nidogen-1 is made by mesenchymal cells and is incorporated into the epithelial BM. It is a sulfated glycoprotein of 150 kDa with three globular domains, G1, G2 and G3, and contains binding sites for other ECM molecules (PMID: 10958695, 10671374).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, rat\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag4749 Product name: Recombinant human NID1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 28-400 aa of BC045606 Sequence: CLSRQELFPFGPGQGDLELEDGDDFVSPALELRGALRFYDRSDIDAVYVTTNGIIATSEPPAKESHPGLFPPTFGAVAPFLADLDTTDGLGKVYYREDLSPSITQRAAECVHRGFPEISFQPSSAVVVTWESVAPYQGPSRDPDQKGKRNTFQAVLASSDSSSYAIFLYPEDGLQFHTTFSKKENNQVPAVVAFSQGSVGFLWKSNGAYNIFANDRESIENLAKSSNSGQQGVWVFEIGSPATTNGVVPADVILGTEDGAEYDDEDEDYDLATTRLGLEDVGTTPFSYKALRRGGADTYSVPSVLSPRRAATERPLGPPTERTRSFQLAVETFHQQHPQVIDVDEVEETGVVFSYNTDSRQTCANNRHQCSVH Predict reactive species\nFull Name: nidogen 1\nCalculated Molecular Weight: 1247 aa, 136 kDa\nObserved Molecular Weight: 136-150 kDa\nGenBank Accession Number: BC045606\nGene Symbol: Entactin\/NID\nGene ID (NCBI): 4811\nRRID: AB_10642564\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: P14543\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46869405106345,"sku":"13766-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-13766-1-ap","provider":"Iright","version":"1.0","type":"link"}