{"product_id":"proteintech-13801-1-ap","title":"Proteintech, 13801-1-AP, Serpin E1\/PAI-1 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe Serpin E1\/PAI-1 (13801-1-AP) by Proteintech is a Polyclonal antibody targeting Serpin E1\/PAI-1 in WB, IHC, IF\/ICC, IP, ELISA applications with reactivity to human samples\n13801-1-AP targets Serpin E1\/PAI-1 in WB, IHC, IF\/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HepG2 cells, HuH-7 cells,  L02 cells,  MG-63 cells,  U2OS cells,  human placenta tissue\nPositive IP detected in: HUVEC cells\nPositive IHC detected in: human placenta tissue, human lung cancer tissue,  human malignant melanoma tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: HepG2 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:1000-1:4000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:200-1:800\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:200-1:800\n\u003cb\u003eBackground Information\u003c\/b\u003e\nSERPINE1, also named as Plasminogen activator inhibitor type 1 (PAI-1), is a member of the serine protease inhibitor (SERPIN) superfamily. PAI-1 is the principal inhibitor of tPA and uPA, and hence is an inhibitor of fibrinolysis. Defects in this gene are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1 deficiency), and high concentrations of the gene product are associated with thrombophilia. In western blotting, we got two bands 50 kDa and 90-110 kDa, and  90-110 kDa consistent with PAI-1 in complex with its target protease, t-PA (PMID: 21596853).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human\nCited Reactivity: human, rabbit, bovine\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag4777 Product name: Recombinant human SERPINE1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-402 aa of BC010860 Sequence: MQMSPALTCLVLGLALVFGEGSAVHHPPSYVAHLASDFGVRVFQQVAQASKDRNVVFSPYGVASVLAMLQLTTGGETQQQIQAAMGFKIDDKGMAPALRHLYKELMGPWNKDEISTTDAIFVQRDLKLVQGFMPHFFRLFRSTVKQVDFSEVERARFIINDWVKTHTKGMISNLLGKGAVDQLTRLVLVNALYFNGQWKTPFPDSSTHRRLFHKSDGSTVSVPMMAQTNKFNYTEFTTPDGHYYDILELPYHGDTLSMFIAAPYEKEVPLSALTNILSAQLISHWKGNMTRLPRLLVLPKFSLETEVDLRKPLENLGMTDMFRQFQADFTSLSDQEPLHVAQALQKVKIEVNESGTVASSSTAVIVSARMAPEEIIMDRPFLFVVRHNPTGTVLFMGQVMEP Predict reactive species\nFull Name: serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1\nCalculated Molecular Weight: 402 aa, 45 kDa\nObserved Molecular Weight: 45 kDa\nGenBank Accession Number: BC010860\nGene Symbol: PAI-1\nGene ID (NCBI): 5054\nRRID: AB_2186881\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: P05121\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868833370281,"sku":"13801-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-13801-1-ap","provider":"Iright","version":"1.0","type":"link"}