{"product_id":"proteintech-13889-1-ap","title":"Proteintech, 13889-1-AP, DHH Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe DHH (13889-1-AP) by Proteintech is a Polyclonal antibody targeting DHH in WB, ELISA applications with reactivity to human, mouse, rat samples\n13889-1-AP targets DHH in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: mouse skeletal muscle tissue\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:2000\n\u003cb\u003eBackground Information\u003c\/b\u003e\nDesert hedgehog (DHH) is one of three ligands in the hedgehog signaling pathway that controls gonadmorphogenesis and nerve perineurium differentiation.What is the molecular weight of DHH? Is DHH post-translationally modified?DHH protein is synthesized in a precursor form of ~45 kDa. Hedgehog proteins contain asmall N-terminal signalpeptide, which iscleaved off intracellularly, leading to 39 kDa species. Hedgehog proteins are then subject toautoproteolysis, which leads to the generation of a 19 kDa N-terminal fragment with added cholesterol moietyand a 25 kDa C-terminal fragment (PMID: 7985023). The C-terminal fragment catalyzes the precursor proteolysis,while theN-terminal fragment gets secreted from cells and acts as an active ligand in the hedgehog signalingpathway.What is the subcellular localization of DHH?DHH protein is subject to autoproteolysis, as described above, and the N-terminal fragment is secreted fromcells in a regulated manner. The amounts of extracellular hedgehog proteins determine local and long-rangesignaling (PMID: 7736596 and 7885476).What is the tissue expression pattern of DHH?Unlike other hedgehog ligands, expression of DHH is very tissue-specific. DHH is strongly expressed in Sterolicellprecursors,adult sterol cells, and in Leydig cells in testis (PMID: 8805249, 12050120)but also ingranulosacells in ovaries (PMID: 15878962), as well as in Schwann cells-glia of the peripheral nervous system(PMID: 7589793).What is the role of DHH in morphogenesis?DHH plays a role in the correct development of male gonads and perineurium. Loss of DHH leads to peripheralneuropathy and gonadal dysgenesis (PMID: 11017805).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, rat\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag4850 Product name: Recombinant human DHH protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 51-396 aa of BC033507 Sequence: GVPERTLGASGPAEGRVARGSERFRDLVPNYNPDIIFKDEENSGADRLMTERCKERVNALAIAVMNMWPGVRLRVTEGWDEDGHHAQDSLHYEGRALDITTSDRDRNKYGLLARLAVEAGFDWVYYESRNHVHVSVKADNSLAVRAGGCFPGNATVRLWSGERKGLRELHRGDWVLAADASGRVVPTPVLLFLDRDLQRRASFVAVETEWPPRKLLLTPWHLVFAARGPAPAPGDFAPVFARRLRAGDSVLAPGGDALRPARVARVAREEAVGVFAPLTAHGTLLVNDVLASCYAVLESHQWAHRAFAPLRLLHALGALLPGGAVQPTGMHWYSRLLYRLAEELLG Predict reactive species\nFull Name: desert hedgehog homolog (Drosophila)\nCalculated Molecular Weight: 396 aa, 44 kDa\nObserved Molecular Weight: 42 kDa\nGenBank Accession Number: BC033507\nGene Symbol: DHH\nGene ID (NCBI): 50846\nRRID: AB_2814660\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: O43323\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46869402943657,"sku":"13889-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-13889-1-ap","provider":"Iright","version":"1.0","type":"link"}