{"product_id":"proteintech-14049-1-ap","title":"Proteintech, 14049-1-AP, MAN1A2 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe MAN1A2 (14049-1-AP) by Proteintech is a Polyclonal antibody targeting MAN1A2 in WB, IP, IHC, ELISA applications with reactivity to human samples\n14049-1-AP targets MAN1A2 in WB, IP, IHC, ELISA applications and shows reactivity with human samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: human placenta tissue\nPositive IP detected in: human placenta tissue\nPositive IHC detected in: human placenta tissue,  human heart tissue,  human lung tissue,  human ovary tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:200-1:1000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:20-1:200\n\u003cb\u003eBackground Information\u003c\/b\u003e\nThe first group or Class I mannosidase is comprised of α1-2 mannosidases from mammals and yeast with encoded polypeptides of 65-75 kDa. Most of them are Ca2+-dependent enzymes located in the ER and\/or Golgi which are inhibited by substrate mimics like kifunensine and deoxymannojirimycin. Their specificity is restricted to α(1-2) linkages, and they are mostly inactive towards artificial substrates like arylmannosides(PMID:10207175).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human\nCited Reactivity: human\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag5240 Product name: Recombinant human MAN1A2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 292-641 aa of BC063300 Sequence: VQLAEKLLPAFNTPTGIPWAMVNLKSGVGRNWGWASAGSSILAEFGTLHMEFIHLSYLTGDLTYYKKVMHIRKLLQKMDRPNGLYPNYLNPRTGRWGQYHTSVGGLGDSFYEYLLKAWLMSDKTDHEARKMYDDAIEAIEKHLIKKSRGGLTFIGEWKNGHLEKKMGHLACFAGGMFALGADGSRADKAGHYLELGAEIARTCHESYDRTALKLGPESFKFDGAVEAVAVRQAEKYYILRPEVIETYWYLWRFTHDPRYRQWGWEAALAIEKYCRVNGGFSGVKDVYSSTPTHDDVQQSFFLAETLKYLYLLFSGDDLLPLDHWVFNTEAHPLPVLHLANTTLSGNPAVR Predict reactive species\nFull Name: mannosidase, alpha, class 1A, member 2\nCalculated Molecular Weight: 641 aa, 73 kDa\nObserved Molecular Weight: 73 kDa\nGenBank Accession Number: BC063300\nGene Symbol: MAN1A2\nGene ID (NCBI): 10905\nRRID: AB_2266033\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: O60476\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46869706735785,"sku":"14049-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-14049-1-ap","provider":"Iright","version":"1.0","type":"link"}