{"product_id":"proteintech-14052-1-ap","title":"Proteintech, 14052-1-AP, CDK6 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe CDK6 (14052-1-AP) by Proteintech is a Polyclonal antibody targeting CDK6 in WB, IHC, IF\/ICC, FC (Intra), IP, ELISA applications with reactivity to human, mouse, rat samples\n14052-1-AP targets CDK6 in WB, IHC, IF\/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: Jurkat cells, C2C12 cells,  MOLT-4 cells,  NIH\/3T3 cells,  K-562 cells\nPositive IP detected in: Jurkat cells\nPositive IHC detected in: human urothelial carcinoma tissue, human endometrial cancer tissue,  human lung cancer tissue,  mouse colon tissue,  rat testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: HeLa cells\nPositive FC (Intra) detected in: HeLa cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:1000-1:8000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:250-1:1000\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:200-1:800\nFlow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension\n\u003cb\u003eBackground Information\u003c\/b\u003e\nCyclin-dependent kinase 6 (CDK6) is an enzyme belonging to the CDK family. CDK6 is a catalyticsubunit of aproteinkinase complex that is essential for progression through the G1 phase of thecell cycle and G1\/S transition,thusactivating cell proliferation. CDK6 partners with cyclins tophosphorylate the retinoblastoma (pRb) protein toreleaseits binding partner E2F; a transcriptionfactor that drives gene expression necessary for DNA replication(PMID: 8114739). CDK6 was longthought of as a redundant homolog of CDK4 as they perform similar roles in cellcycle progression.But recently it has become apparent that CDK6 and CDK4 differ intissue-specific functions,whereCDK6 plays a role in differentiation (PMID: 16410727) but also in contribution to tumor development.What is the molecular weight of CDK6?The molecular weight of CDK6 is approximately 36-40 kDa (PMID: 23563707, 8114739).What is the subcellular localization of CDK6?CDK6 is ubiquitously expressed through the nucleus and cytoplasm. However, the most active complexes arelocatedin the nuclei of proliferating cells.What is the expression pattern of CDK6?CDK6 activity first occurs midway through the G1 phase. Its expression isregulated by D-typecyclins andmembersoftheINK4 family of CDK inhibitors (PMID: 7739547).What is CDK6's role in cancer?CDK6 is involved in a critical regulatory point in the cell cycle and so is found to be unbalanced in approximately80-90% of tumors (PMID: 23356980). Loss of cell cycle control is the initial step in the development ofthehallmarksof cancer, such as sustained proliferative signaling and dysregulated cellular energetics. Deregulationof CDK6 has animportant role in lymphoma by increasing angiogenesis (PMID: 23948297) and upregulated CDK6expression is alsoassociated with a poor clinical prognosis in medulloblastoma (PMID: 23172372).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, mouse, rat, zebrafish, goat\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag5275 Product name: Recombinant human CDK6 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-326 aa of BC027989 Sequence: MEKDGLCRADQQYECVAEIGEGAYGKVFKARDLKNGGRFVALKRVRVQTGEEGMPLSTIREVAVLRHLETFEHPNVVRLFDVCTVSRTDRETKLTLVFEHVDQDLTTYLDKVPEPGVPTETIKDMMFQLLRGLDFLHSHRVVHRDLKPQNILVTSSGQIKLADFGLARIYSFQMALTSVVVTLWYRAPEVLLQSSYATPVDLWSVGCIFAEMFRRKPLFRGSSDVDQLGKILDVIGLPGEEDWPRDVALPRQAFHSKSAQPIEKFVTDIDELGKDLLLKCLTFNPAKRISAYSALSHPYFQDLERCKENLDSHLPPSQNTSELNTA Predict reactive species\nFull Name: cyclin-dependent kinase 6\nCalculated Molecular Weight: 326 aa, 36 kDa\nObserved Molecular Weight: 36-40 kDa\nGenBank Accession Number: BC027989\nGene Symbol: CDK6\nGene ID (NCBI): 1021\nRRID: AB_10642144\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q00534\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868822884521,"sku":"14052-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-14052-1-ap","provider":"Iright","version":"1.0","type":"link"}