{"product_id":"proteintech-14073-1-ap","title":"Proteintech, 14073-1-AP, SRPK1 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe SRPK1 (14073-1-AP) by Proteintech is a Polyclonal antibody targeting SRPK1 in WB, IHC, IF\/ICC, IP, ELISA applications with reactivity to human, mouse samples\n14073-1-AP targets SRPK1 in WB, IHC, IF\/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HeLa cells, Jurkat cells,  L02 cells,  mouse testis tissue\nPositive IP detected in: mouse testis tissue\nPositive IHC detected in: human breast cancer tissue,  human testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: SH-SY5Y cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:1000-1:4000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:20-1:200\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:10-1:100\n\u003cb\u003eBackground Information\u003c\/b\u003e\nSRPK1 (serine-arginine protein kinase 1) is a protein kinase that specifically phosphorylates proteins containing serine-arginine-rich domains. Its substrates include a family of SR proteins that are key regulators of mRNA AS (alternative splicing). SRPK1 may be localized both in the cytoplasm and nucleus and translocates between these compartments under various conditions (e.g. cell cycle and stress). It is able to phosphorylate SR proteins both in cytoplasm and nucleus (the significance of which remains incompletely understood) and thus regulate the activity of these proteins by affecting their ability to bind other proteins and\/or RNA as well as their intracellular localization.(PMID:22817743). This protein has 2 isoforms produced by alternative splicing.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse\nCited Reactivity: human, mouse, chicken\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag5149 Product name: Recombinant human SRPK1 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 306-655 aa of BC038292 Sequence: QEESESPVERPLKENPPNKMTQEKLEESSTIGQDQTLMERDTEGGAAEINCNGVIEVINYTQNSNNETLRHKEDLHNANDCDVQNLNQESSFLSSQNGDSSTSQETDSCTPITSEVSDTMVCQSSSTVGQSFSEQHISQLQESIRAEIPCEDEQEQEHNGPLDNKGKSTAGNFLVNPLEPKNAEKLKVKIADLGNACWVHKHFTEDIQTRQYRSLEVLIGSGYNTPADIWSTACMAFELATGDYLFEPHSGEEYTRDEDHIALIIELLGKVPRKLIVAGKYSKEFFTKKGDLKHITKLKPWGLFEVLVEKYEWSQEEAAGFTDFLLPMLELIPEKRATAAECLRHPWLNS Predict reactive species\nFull Name: SFRS protein kinase 1\nCalculated Molecular Weight: 92.4 kDa\nObserved Molecular Weight: 72-95 kDa\nGenBank Accession Number: BC038292\nGene Symbol: SRPK1\nGene ID (NCBI): 6732\nRRID: AB_2194745\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q96SB4\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868930396329,"sku":"14073-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-14073-1-ap","provider":"Iright","version":"1.0","type":"link"}