{"product_id":"proteintech-14245-1-ap","title":"Proteintech, 14245-1-AP, BIN2 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe BIN2 (14245-1-AP) by Proteintech is a Polyclonal antibody targeting BIN2 in WB, IP, IHC, ELISA applications with reactivity to human, mouse samples\n14245-1-AP targets BIN2 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: Jurkat cells, U-937 cells\nPositive IP detected in: Jurkat cells\nPositive IHC detected in: human spleen tissue, mouse heart tissue,  human heart tissue,  human skin tissue,  human lung tissue,  human ovary tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:2000-1:14000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:20-1:200\n\u003cb\u003eBackground Information\u003c\/b\u003e\nBAR proteins, which are characterized by a common N-terminal BAR domain, are a family of adaptor proteins implicated in a diverse set of cellular processes. BIN1 (bridging integrator 1) and BIN2 are BAR proteins. The human BIN2 gene is located at chromosome 4q22.1, and encodes a 565-amino acid protein with a predicted molecular weight of ~62 kDa. It shows preferential expression in hematopoietic tissues, with highest mRNA levels in spleen and peripheral blood leukocytes and also high levels in thymus, colon, and placenta. BIN2 has been shown to migrate with an apparent mobility of ~80 kDa, and interact with BIN1. (PMID: 10903846)\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse\nCited Reactivity: mouse\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag5502 Product name: Recombinant human BIN2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 216-565 aa of BC047686 Sequence: LRDVFYREMSKLNHNLYEVMSKLEKQHSNKVFVVKGLSSSSRRSLVISPPVRTATVSSPLTSPTSPSTLSLKSESESVSATEDLAPDAAQGEDNSEIKELLEEEEIEKEGSEASSSEEDEPLPACNGPAQAQPSPTTERAKSQEEVLPSSTTPSPGGALSPSGQPSSSATEVVLRTRTASEGSEQPKKRASIQRTSAPPSRPPPPRATASPRPSSGNIPSSPTASGGGSPTSPRASLGTGTASPRTSLEVSPNPEPPEKPVRTPEAKENENIHNQNPEELCTSPTLMTSQVASEPGEAKKMEDKEKDNKLISADSSEGQDQLQVSMVPENNNLTAPEPQEEVSTSENPQL Predict reactive species\nFull Name: bridging integrator 2\nCalculated Molecular Weight: 62 kDa\nObserved Molecular Weight: 80-90 kDa\nGenBank Accession Number: BC047686\nGene Symbol: BIN2\nGene ID (NCBI): 51411\nRRID: AB_2063647\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q9UBW5\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46869642903721,"sku":"14245-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-14245-1-ap","provider":"Iright","version":"1.0","type":"link"}