{"product_id":"proteintech-14268-1-ap","title":"Proteintech, 14268-1-AP, BMAL1 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe BMAL1 (14268-1-AP) by Proteintech is a Polyclonal antibody targeting BMAL1 in WB, IF\/ICC, FC (Intra), IP, ELISA applications with reactivity to human, mouse, rat samples\n14268-1-AP targets BMAL1 in WB, IHC, IF\/ICC, FC (Intra), IP, CoIP, chIP, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HT-1080 cells, 3T3-L1 cells,  HeLa cells,  SH-SY5Y cells,  mouse liver tissue,  MCF-7 cells,  NCI-H1299 cells,  rat liver tissue\nPositive IP detected in: HeLa cells\nPositive IF\/ICC detected in: U-87 MG cells\nPositive FC (Intra) detected in: HeLa cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:2000-1:10000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:50-1:500\nFlow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension\n\u003cb\u003eBackground Information\u003c\/b\u003e\nBMAL1, a basic helix-loop-helices with Per-Arnt-Sim (PAS) domain transcription factor, is one of components that has core clock function in mammals. It form a heterodimer with CLOCK, and induce the transcription of target genes by binding to E-box elements within their promoter regions. And this transcription is inhibited in a feedback loop by PER, and also by CRY proteins.Also BMAL1 is a key player in the regulation of metabolism, playing a role in adipogenesis and in the control of glucose and triglycerides levels. Due to phosphorylation modification, BMAL1 can be detected as a band in the range of 70-80 kda (PMID: 14750952).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, mouse, rat\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag5586 Product name: Recombinant human ARNTL protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-303 aa of BC041129 Sequence: MADQRMDISSTISDFMSPGPTDLLSSSLGTSGVDCNRKRKGSSTDYQESMDTDKDDPHGRLEYTEHQGRIKNAREAHSQIEKRRRDKMNSFIDELASLVPTCNAMSRKLDKLTVLRMAVQHMKTLRGATNPYTEANYKPTFLSDDELKHLILRAADGFLFVVGCDRGKILFVSESVFKILNYSQNDLIGQSLFDYLHPKDIAKVKEQLSSSDTAPRERLIDAKTGLPVKTDITPGPSRLCSGARRSFFCRMKCNRPSVKVEDKDFPSTCSKKKDRKSFCTIHSTGYLKSWPPTKMGLDEDNEP Predict reactive species\nFull Name: aryl hydrocarbon receptor nuclear translocator-like\nCalculated Molecular Weight: 69 kDa\nObserved Molecular Weight: 69-75 kDa\nGenBank Accession Number: BC041129\nGene Symbol: ARNTL\nGene ID (NCBI): 406\nRRID: AB_2878037\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: O00327\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868832911529,"sku":"14268-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-14268-1-ap","provider":"Iright","version":"1.0","type":"link"}