{"product_id":"proteintech-14274-1-ap","title":"Proteintech, 14274-1-AP, GFM1 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe GFM1 (14274-1-AP) by Proteintech is a Polyclonal antibody targeting GFM1 in WB, IHC, IF\/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples\n14274-1-AP targets GFM1 in WB, IHC, IF\/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: CTLL-2 cells, HEK-293T cells,  HeLa cells,  SKOV-3 cells,  mouse heart tissue,  rat heart tissue\nPositive IP detected in: HeLa cells\nPositive IHC detected in: human kidney tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: HeLa cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:1000-1:4000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:20-1:200\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nDifferent factors catalyze the three stages of protein translation: initiation, elongation, and termination. There are two translational systems in eukaryotes, one in the cytoplasm and the other in the mitochondria. In mitochondria, the elongation phase requires three elongation factors (EF): Tu (TUFM), Ts (TSFM), and G (GFM1)[PMID:19716793]. GFM1 catalyzes translocation during peptide elongation and mediates ribosomal disassembly during ribosome recycling in concert with the ribosomal recycling factor (RRF). [PMID:16487710]\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, mouse\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag5616 Product name: Recombinant human GFM1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 400-751 aa of BC049210 Sequence: RMHADMMEDVEEVYAGDICALFGIDCASGDTFTDKANSGLSMESIHVPDPVISIAMKPSNKNDLEKFSKGIGRFTREDPTFKVYFDTENKETVISGMGELHLEIYAQRLEREYGCPCITGKPKVAFRETITAPVPFDFTHKKQSGGAGQYGKVIGVLEPLDPEDYTKLEFSDETFGSNIPKQFVPAVEKGFLDACEKGPLSGHKLSGLRFVLQDGAHHMVDSNEISFIRAGEGALKQALANATLCILEPIMAVEVVAPNEFQGQVIAGINRRHGVITGQDGVEDYFTLYADVPLNDMFGYSTELRSCTEGKGEYTMEYSRYQPCLPSTQEDVINKYLEATGQLPVKKGKAKN Predict reactive species\nFull Name: G elongation factor, mitochondrial 1\nCalculated Molecular Weight: 86 kDa\nObserved Molecular Weight: 85 kDa\nGenBank Accession Number: BC049210\nGene Symbol: GFM1\nGene ID (NCBI): 85476\nRRID: AB_2110140\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q96RP9\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46869407924393,"sku":"14274-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-14274-1-ap","provider":"Iright","version":"1.0","type":"link"}