{"product_id":"proteintech-14773-1-ap","title":"Proteintech, 14773-1-AP, TARS Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe TARS (14773-1-AP) by Proteintech is a Polyclonal antibody targeting TARS in WB, IHC, IF\/ICC, IP, ELISA applications with reactivity to human samples\n14773-1-AP targets TARS in WB, IHC, IF\/ICC, IP, ELISA applications and shows reactivity with human samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: A549 cells\nPositive IP detected in: A549 cells\nPositive IHC detected in: human breast cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: MCF-7 cells,  Hela cells,  HepG2 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:1000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:100-1:400\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:10-1:100\n\u003cb\u003eBackground Information\u003c\/b\u003e\nTARS (Threonyl-tRNA synthetase), also known as threonine-tRNA ligase or ThrRS, belongs to the aminoacyl-tRNA synthetase family. Aminoacyl-tRNA synthetase (aaRS) catalyzes the specific esterification of an amino acid with the 3'-terminal hydroxyl group of its cognate tRNA. Specificity of aminoacylation catalyzed by aaRSs ensures the accurate translation of the genetic code in the first step of protein synthesis (PMID: 26324710). TARS has some isoforms with the molecular mass of 83, 87 kDa and other MW.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human\nCited Reactivity: human\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag6614 Product name: Recombinant human TARS protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 394-723 aa of BC000517 Sequence: ENMFSFEVEKELFALKPMNCPGHCLMFDHRPRSWRELPLRLADFGVLHRNELSGALTGLTRVRRFQQDDAHIFCAMEQIEDEIKGCLDFLRTVYSVFGFSFKLNLSTRPEKFLGDIEVWDQAEKQLENSLNEFGEKWELNSGDGAFYGPKIDIQIKDAIGRYHQCATIQLDFQLPIRFNLTYVSHDGDDKKRPVIVHRAILGSVERMIAILTENYGGKWPFWLSPRQVMVVPVGPTCDEYAQKVRQQFHDAKFMADIDLDPGCTLNKKIRNAQLAQYNFILVVGEKEKISGTVNIRTRDNKVHGERTISETIERLQQLKEFRSKQAEEEF Predict reactive species\nFull Name: threonyl-tRNA synthetase\nCalculated Molecular Weight: 83 kDa\nObserved Molecular Weight: 66-72 kDa\nGenBank Accession Number: BC000517\nGene Symbol: TARS\nGene ID (NCBI): 6897\nRRID: AB_2200654\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: P26639\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46869503279273,"sku":"14773-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-14773-1-ap","provider":"Iright","version":"1.0","type":"link"}