{"product_id":"proteintech-14914-1-ap","title":"Proteintech, 14914-1-AP, HN1 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe HN1 (14914-1-AP) by Proteintech is a Polyclonal antibody targeting HN1 in WB, IP, IHC, ELISA applications with reactivity to human, mouse, rat samples\n14914-1-AP targets HN1 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HeLa cells, human brain tissue,  human testis tissue,  HepG2 cells,  A431 cells,  LNCaP cells,  mouse testis tissue,  mouse colon tissue\nPositive IP detected in: MCF-7 cells\nPositive IHC detected in: human ovary tumor tissue, human breast cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:1000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nHN1 (hematopoietic- and neurologic-expressed sequence 1) is a highly conserved protein that is expressed in developing and regenerating tissues. Overexpression of HN1 has been reported in various tumors like glioma and breast cancer. Hn1 has also been reported as a marker for human ovarian carcinoma and can distinguish epithelial ovarian carcinoma cells from normal ovarian surface epithelial cells. This collective information suggests that HN1 is involved in processes associated with cell proliferation, repair and\/or growth.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, mouse\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag6712 Product name: Recombinant human HN1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-154 aa of BC001420 Sequence: MTTTTTFKGVDPNSRNSSRVLRPPGGGSNFSLGFDEPTEQPVRKNKMASNIFGTPEENQASWAKSAGAKSSGGREDLESSGLQRRNSSEASSGDFLDLKGEGDIHENVDTDLPGSLGQSEEKPVPAAPVPSPVAPAPVPSRRNPPGGKSSLVLG Predict reactive species\nFull Name: hematological and neurological expressed 1\nCalculated Molecular Weight: 11 kDa, 16 kDa, 19 kDa\nObserved Molecular Weight: 23-30 kDa\nGenBank Accession Number: BC001420\nGene Symbol: HN1\nGene ID (NCBI): 51155\nRRID: AB_10863118\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q9UK76\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868955660457,"sku":"14914-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-14914-1-ap","provider":"Iright","version":"1.0","type":"link"}