{"product_id":"proteintech-14948-1-ap","title":"Proteintech, 14948-1-AP, LMOD3 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe LMOD3 (14948-1-AP) by Proteintech is a Polyclonal antibody targeting LMOD3 in WB, IHC, IP, ELISA applications with reactivity to human, mouse, rat samples\n14948-1-AP targets LMOD3 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: mouse skeletal muscle tissue, mouse heart tissue,  rat skeletal muscle tissue\nPositive IP detected in: mouse skeletal muscle tissue\nPositive IHC detected in: mouse skeletal muscle tissue, human heart tissue,  human skeletal muscle tissue,  mouse heart tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:2000-1:10000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:500-1:2000\n\u003cb\u003eBackground Information\u003c\/b\u003e\nThe gene encoding LMOD3 has not been characterized so far and very limited information of its function has been reported. Nanda et al. found that the expression of mouse LMOD3 mRNA is restricted largely to cardiac and skeletal muscle through RT-PCR analysis (PMID: 22157009). Two isoforms of LMOD3 may exist due to the alternative splicing, whose molecular weights are predicted as 65 kDa and 40 kDa, respectively (Uniprot). This antibody was raised against the N-terminal region of human LMOD3. It detects a double bands around 80 kDa and 65 kDa in heart and skeletal muscle lysates. The reason causing the discrepancy between the predicted and observed molecular weight is not clear.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, mouse, zebrafish, xenopus\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag6758 Product name: Recombinant human LMOD3 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-236 aa of BC039202 Sequence: MSEHSRNSDQEELDEEINEDEILANLSAEELKELQSEMEVMAPDPSLPVGMIQKDQTDKPPTGNFNHKSLVDYMYWEKASRRMLEEERVPVTFVKSEEKTQEEHEEIEKRNKNMAQYLKEKLNNEIVANKRESKGSSNIQETDEEDEEEEDDDDDDEGEDDGEESEETNREEEGKAKEQIRNCENNCQQVTDKAFKEQRDRPEAQEKKKKKISQGKIIFRKNNVRAQQKFRSRRTR Predict reactive species\nFull Name: leiomodin 3 (fetal)\nCalculated Molecular Weight: 65 kDa\nObserved Molecular Weight: 65-70 kDa\nGenBank Accession Number: BC039202\nGene Symbol: LMOD3\nGene ID (NCBI): 56203\nRRID: AB_2136690\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q0VAK6\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868885438633,"sku":"14948-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-14948-1-ap","provider":"Iright","version":"1.0","type":"link"}