{"product_id":"proteintech-15143-1-ap","title":"Proteintech, 15143-1-AP, VPS72 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe VPS72 (15143-1-AP) by Proteintech is a Polyclonal antibody targeting VPS72 in WB, IP, IHC, ELISA applications with reactivity to human, mouse, rat samples\n15143-1-AP targets VPS72 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HeLa cells, mouse skeletal muscle tissue,  NIH\/3T3 cells,  A549 cells\nPositive IP detected in: NIH\/3T3 cells\nPositive IHC detected in: human breast hyperplasia tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:1000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:500-1:2000\n\u003cb\u003eBackground Information\u003c\/b\u003e\nThe mammalian TRRAP\/TIP60-containing histone acetyltransferase (HAT) complex, which exists in Drosophila melanogaster and mammalian cells, is a complex that is responsible for various cellular processes, including DNA repair, transcriptional activation and apoptosis. Vacuolar protein sorting-associated protein 72 homolog (VPS72), also known as VPS72 or Transcription factor-like 1, is a 364 amino acid subunit of the TRRAP\/TIP60 HAT complex. VPS72 has also been identified as a subunit of a novel complex containing SNF2-related helicase SRCAP (SWI2\/SNF2-related CBP activator protein). This SRCAP-containing complex is very similar to the S. cerevisiae SWR1 chromatin remodeling complex. The involvement of VPS72 in these complexes has suggested that VPS72 plays multiple roles in chromatin modification and remodeling in cells. VPS72 localizes to the nucleus and is phosphorylated upon DNA damage, most likely by ATM or ATR.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag7424 Product name: Recombinant human VPS72 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-364 aa of BC003151 Sequence: MSLAGGRAPRKTAGNRLSGLLEAEEEDEFYQTTYGGFTEESGDDEYQGDQSDTEDEVDSDFDIDEGDEPSSDGEAEEPRRKRRVVTKAYKEPLKSLRPRKVNTPAGSSQKAREEKALLPLELQDDGSDSRKSMRQSTAEHTRQTFLRVQERQGQSRRRKGPHCERPLTQEELLREAKITEELNLRSLETYERLEADKKKQVHKKRKCPGPIITYHSVTVPLVGEPGPKEENVDIEGLDPAPSVSALTPHAGTGPVNPPARCSRTFITFSDDATFEEWFPQGRPPKVPVREVCPVTHRPALYRDPVTDIPYATARAFKIIREAYKKYITAHGLPPTASALGPGPPPPEPLPGSGPRALRQKIVIK Predict reactive species\nFull Name: vacuolar protein sorting 72 homolog (S. cerevisiae)\nCalculated Molecular Weight: 41 kDa\nObserved Molecular Weight: 57 kDa\nGenBank Accession Number: BC003151\nGene Symbol: VPS72\nGene ID (NCBI): 6944\nRRID: AB_2215534\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q15906\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46869508817065,"sku":"15143-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-15143-1-ap","provider":"Iright","version":"1.0","type":"link"}