{"product_id":"proteintech-15255-1-ap","title":"Proteintech, 15255-1-AP, H6PD Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe H6PD (15255-1-AP) by Proteintech is a Polyclonal antibody targeting H6PD in WB, IP, IHC, ELISA applications with reactivity to human, mouse, rat samples\n15255-1-AP targets H6PD in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HepG2 cells, mouse liver tissue,  HeLa cells\nPositive IP detected in: HepG2 cells\nPositive IHC detected in: human hepatocirrhosis tissue,  human liver cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:2000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:20-1:200\n\u003cb\u003eBackground Information\u003c\/b\u003e\nHexose-6-phosphate dehydrogenase (H6PD) is also named as GDH,6PGL. It is the main NADPH generating enzyme in the lumen of the endoplasmic reticulum. H6PD is regarded as an ancillary enzyme in prereceptorial glucocorticoid activation and probably acts as a nutrient sensor and as a prosurvival factor(PMID:21620971). H6PD is a glycoprotein and has been shown tissue differences in glycosylation status might explain both the difference in migration on SDS-PAGE gels as well as in activity. Defects in H6PD are a cause of cortisone reductase deficiency (CRD). This antibody is specific to H6PD.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, mouse, rat\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag7109 Product name: Recombinant human H6PD protein Source: e coli. -derived, T-HIS Tag: 6*His Domain: 443-791 aa of BC081559 Sequence: LSDYYAYSPVQERDAHSVLLSHIFHGRKNFFITTENLLASWNFWTPLLESLAHKAPRLYPGGAENGRLLDFEFSSGRLFFSQQQPEQLVPGPGPAPMPSDFQVLRAKYRESPLVSAWSEELISKLANDIEATAVRAVRRFGQFHLALSGGSSPVALFQQLATAHYGFPWAHTHLWLVDERCVPLSDPESNFQGLQAHLLQHVRIPYYNIHPMPVHLQQRLCAEEDQGAQIYAREISALVANSSFDLVLLGMGADGHTASLFPQSPTGLDGEQLVVLTTSPSQPHRRMSLSLPLINRAKKVAVLVMGRMKREITTLVSRVGHEPKKWPISGVLPHSGQLVWYMDYDAFLG Predict reactive species\nFull Name: hexose-6-phosphate dehydrogenase (glucose 1-dehydrogenase)\nCalculated Molecular Weight: 89 kDa\nObserved Molecular Weight: 89-95 kDa\nGenBank Accession Number: BC081559\nGene Symbol: H6PD\nGene ID (NCBI): 9563\nRRID: AB_10642699\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: O95479\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46869464154281,"sku":"15255-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-15255-1-ap","provider":"Iright","version":"1.0","type":"link"}