{"product_id":"proteintech-15817-1-ap","title":"Proteintech, 15817-1-AP, XRCC4 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe XRCC4 (15817-1-AP) by Proteintech is a Polyclonal antibody targeting XRCC4 in WB, IHC, IP, ELISA applications with reactivity to human, rat samples\n15817-1-AP targets XRCC4 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HepG2 cells, HEK-293 cells,  COLO 320 cells\nPositive IP detected in: HepG2 cells\nPositive IHC detected in: human breast cancer tissue, rat testis tissue,  rat small intestine tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:2000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nThere are at least two pathways for eukaryotes to repair DNA double-strand breaks: homologous recombination and nonhomologous end joining(NHEJ). XRCC4 is one of the core machiney of NHEJ that required for double-strand break repair and V(D)J recombination. The DNA ligase IV(LIG4)-XRCC4 complex is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of LIG4.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, rat\nCited Reactivity: human, mouse\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag8606 Product name: Recombinant human XRCC4 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 6-310 aa of BC005259 Sequence: SRIHLVSEPSITHFLQVSWEKTLESGFVITLTDGHSAWTGTVSESEISQEADDMAMEKGKYVGELRKALLSGAGPADVYTFNFSKESCYFFFEKNLKDVSFRLGSFNLEKVENPAEVIRELICYCLDTIAENQAKNEHLQKENERLLRDWNDVQGRFEKCVSAKEALETDLYKRFILVLNEKKTKIRSLHNKLLNAAQEREKDIKQEGETAICSEMTADRDPVYDESTDEESENQTDLSGLASAAVSKDDSIISSLDVTDIAPSRKRRQRMQRNLGTEPKMAPQENQLQEKEKGRKKETSEKEAV Predict reactive species\nFull Name: X-ray repair complementing defective repair in Chinese hamster cells 4\nCalculated Molecular Weight: 310 aa, 35 kDa\nObserved Molecular Weight: 55 kDa\nGenBank Accession Number: BC005259\nGene Symbol: XRCC4\nGene ID (NCBI): 7518\nRRID: AB_2257478\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q13426\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868895826089,"sku":"15817-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-15817-1-ap","provider":"Iright","version":"1.0","type":"link"}