{"product_id":"proteintech-15865-1-ap","title":"Proteintech, 15865-1-AP, CAP2 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe CAP2 (15865-1-AP) by Proteintech is a Polyclonal antibody targeting CAP2 in WB, IHC, IF\/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples\n15865-1-AP targets CAP2 in WB, IHC, IF\/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: PC-3 cells, mouse skeletal muscle tissue,  mouse testis tissue,  mouse heart tissue,  HepG2 cells,  mouse kidney tissue,  rat testis tissue,  human kidney tissue\nPositive IP detected in: mouse testis tissue\nPositive IHC detected in: human liver cancer tissue,  human skeletal muscle tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: U2OS cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:1000-1:6000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:20-1:200\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nCAPs (CAP1 and CAP2) are evolutionarily conserved proteins with roles in regulating the actin cytoskeleton and in signal transduction. CAP2 is predominantly expressed in brain, heart and skeletal muscle, and skin. It is found in the nucleus in undifferentiated myoblasts and at the M-line of differentiated myotubes. Overexpression of CAP2 has been reported in many cancers, including hepatocellular carcinoma (HCC), human breast cancer, and malignant melanoma.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, mouse, rat\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag8477 Product name: Recombinant human CAP2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 129-477 aa of BC008481 Sequence: MFNHLSAVSESIPALGWIAVSPKPGPYVKEMNDAATFYTNRVLKDYKHSDLRHVDWVKSYLNIWSELQAYIKEHHTTGLTWSKTGPVASTVSAFSVLSSGPGLPPPPPPLPPPGPPPLFENEGKKEESSPSRSALFAQLNQGEAITKGLRHVTDDQKTYKNPSLRAQGGQTQSPTKSHTPSPTSPKSYPSQKHAPVLELEGKKWRVEYQEDRNDLVISETELKQVAYIFKCEKSTIQIKGKVNSIIIDNCKKLGLVFDNVVGIVEVINSQDIQIQVMGRVPTISINKTEGCHIYLSEDALDCEIVSAKSSEMNILIPQDGDYREFPIPEQFKTAWDGSKLITEPAEIMA Predict reactive species\nFull Name: CAP, adenylate cyclase-associated protein, 2 (yeast)\nCalculated Molecular Weight: 477 aa, 53 kDa\nObserved Molecular Weight: 53 kDa\nGenBank Accession Number: BC008481\nGene Symbol: CAP2\nGene ID (NCBI): 10486\nRRID: AB_2270174\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: P40123\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868947599529,"sku":"15865-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-15865-1-ap","provider":"Iright","version":"1.0","type":"link"}