{"product_id":"proteintech-16010-1-ap","title":"Proteintech, 16010-1-AP, LAS1L Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe LAS1L (16010-1-AP) by Proteintech is a Polyclonal antibody targeting LAS1L in WB, IHC, IF\/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples\n16010-1-AP targets LAS1L in WB, IHC, IF\/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: COLO 320 cells, HEK-293 cells,  HeLa cells,  HepG2 cells,  PC-3 cells\nPositive IP detected in: HEK-293 cells\nPositive IHC detected in: human ovary cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: HepG2 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:3000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:50-1:500\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:20-1:200\n\u003cb\u003eBackground Information\u003c\/b\u003e\nLAS1L is a nucleolar protein required for the synthesis of the 60S ribosomal subunit and maturation of the 28S rRNA. LAS1L interacts with PELP1, TEX10, and WDR18, along with NOL9 and SENP3, to form a novel nucleolar complex that cofractionates with the 60S preribosomal subunit.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag8732 Product name: Recombinant human LAS1L protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-353 aa of BC014545 Sequence: MSWESGAGPGLGSQGMDLVWSAWYGKCVKGKGSLPLSAHGIVVAWLSRAEWDQVTVYLFCDDHKLQRYALNRITVWRSRSGNELPLAVASTADLIRCKLLDVTGGLGTDELRLLYGMALVRFVNLISERKTKFAKVPLKCLAQEVNIPDWIVDLRHELTHKKMPHINDCRRGCYFVLDWLQKTYWCRQLENSLRETWELEEFREGIEEEDQEEDKNIVVDDITEQKPEPQDDGKSTESDVKADGDSKGSEEVDSHCKKALSHKELYERARELLVSYEEEQFTVLEKFRYLPKAIKAWNNPSPRVECVLAELKGVTCENREAVLDAFLDDGFLVPTFEQLAALQIEYEDGQTEV Predict reactive species\nFull Name: LAS1-like (S. cerevisiae)\nCalculated Molecular Weight: 734 aa, 83 kDa\nObserved Molecular Weight: 83 kDa, 70-75 kDa\nGenBank Accession Number: BC014545\nGene Symbol: LAS1L\nGene ID (NCBI): 81887\nRRID: AB_2132810\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q9Y4W2\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46869001666729,"sku":"16010-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-16010-1-ap","provider":"Iright","version":"1.0","type":"link"}