{"product_id":"proteintech-16150-1-ap","title":"Proteintech, 16150-1-AP, BAG1S\/1M\/1L Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe BAG1S\/1M\/1L (16150-1-AP) by Proteintech is a Polyclonal antibody targeting BAG1S\/1M\/1L in WB, IHC, IP, ELISA applications with reactivity to human, mouse samples\n16150-1-AP targets BAG1S\/1M\/1L in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: mouse brain tissue,  Jurkat cells,  mouse liver tissue\nPositive IP detected in: Jurkat cells\nPositive IHC detected in: human testis tissue,  human kidney tissue,  human lung tissue,  human ovary tissue,  human placenta tissue,  human spleen tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:1000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:20-1:200\n\u003cb\u003eBackground Information\u003c\/b\u003e\nBAG1 has been identified that modulates gene transcription through poorly defined mechanisms. Four isoforms of the BAG1 protein (BAG1S, BAG1, BAG1M, and BAG1L) can be produced from a common mRNA by use of alternative translation initiation sites, including a non-canonical CTG codon in one instance. The longest, BAG1L (Mr ~50K), contains a nuclear localization signal (NLS) and resides in the nucleus, whereas BAG1M (Mr ~46K) has an incomplete NLS and distributes mainly in cytosol, unless dragged into the nucleus through interactions with other. Distribution of BAG1S(p33) is not clear yet. This antibody recognizes three isoforms of BAG1, includes GAG1L, BAG1M, and BAG1S.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse\nCited Reactivity: human\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: BCL2-associated athanogene\nCalculated Molecular Weight: 26 kDa\nObserved Molecular Weight: 46-50 kDa, 41-46 kDa, 25-33 kDa\nGenBank Accession Number: NM_004323\nGene Symbol: BAG1\nGene ID (NCBI): 573\nRRID: AB_2062288\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q99933\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46869641789609,"sku":"16150-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-16150-1-ap","provider":"Iright","version":"1.0","type":"link"}