{"product_id":"proteintech-16177-1-ap","title":"Proteintech, 16177-1-AP, Lin28A-specific Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe Lin28A-specific (16177-1-AP) by Proteintech is a Polyclonal antibody targeting Lin28A-specific in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples\n16177-1-AP targets Lin28A-specific in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: hESC cells, human brain tissue,  human heart tissue,  NCCIT cell,  mouse liver tissue\nPositive IHC detected in: human small intestine tissue, human ovary tissue,  human heart tissue,  human testis tissue,  human spleen tissue,  human lung tissue,  human brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:200-1:1000\nImmunohistochemistry (IHC): IHC : 1:20-1:200\n\u003cb\u003eBackground Information\u003c\/b\u003e\nLIN28 is one of the four key human factors (OCT4, SOX2, NANOG and LIN28) used to reprogram human fibroblasts to an embryonic stem (ES) cell-like state known as the induced pluripotent stem (Ips) cell. LIN28 is a marker of undifferentiated human embryonic stem cells and a cytoplasmic mRNA-binding protein that binds to and enhances the translation of the IGF2 mRNA. LIN28 has also been shown to bind to the let-7 pre-miRNA and block production of the mature let-7 microRNA in mouse embryonic stem cells. Affinity purified rabbit anti-LIN28 can be used to demonstrate pluripotency of ES and Ips cells, and to detect LIN28 transgene expression in the process of reprogramming. This antibody is a rabbit polyclonal antibody raised against a peptide mapping within human LIN28.This antibody recognize Lin28A specifically.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, mouse, rat\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: lin-28 homolog (C. elegans)\nCalculated Molecular Weight: 23 kDa\nObserved Molecular Weight: 28 kDa\nGenBank Accession Number: NM_024674\nGene Symbol: LIN28\nGene ID (NCBI): 79727\nRRID: AB_2135038\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q9H9Z2\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868913029289,"sku":"16177-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-16177-1-ap","provider":"Iright","version":"1.0","type":"link"}