{"product_id":"proteintech-16215-1-ap","title":"Proteintech, 16215-1-AP, ACAT1 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe ACAT1 (16215-1-AP) by Proteintech is a Polyclonal antibody targeting ACAT1 in WB, IHC, IF\/ICC, FC (Intra), IP, ELISA applications with reactivity to human, mouse samples\n16215-1-AP targets ACAT1 in WB, IHC, IF\/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: mouse heart tissue, Caco-2 cells,  human skeletal muscle tissue,  MCF-7 cells,  rat skeletal muscle tissue,  mouse colon tissue\nPositive IP detected in: mouse heart tissue\nPositive IHC detected in: human  colon, human liver tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: HeLa cells\nPositive FC (Intra) detected in: HeLa cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:2000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:50-1:500\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:200-1:800\nFlow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension\n\u003cb\u003eBackground Information\u003c\/b\u003e\nACAT1 (acetyl-CoA acetyltransferase), is a mitochondrial enzyme catalyzing the reversible formation or breakdown of acetoacetyl-CoA and plays important roles in ketogenesis and ketolysis. Deficiency of ACAT1 causes an inherited metabolic disorder. Recently ACAT1 has been reported to get involved in cancer progression.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse\nCited Reactivity: human, mouse, rat, rabbit\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag9215 Product name: Recombinant human ACAT1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-162 aa of BC010942 Sequence: MAVLPALLRSGARSRSPLLRRLVQEIRYVERSYVSKPTLKEVVIVSATRTPIGSFLGSLSLLPATKLGSIAIQGAIEKAGIPKEEVKEAYMGNVLQGGEGQAPTRQAVLGAGLPISTPCTTINKVCASGMKAIMMASQSLMCGHQIKQETGSLAKICCHVRR Predict reactive species\nFull Name: acetyl-Coenzyme A acetyltransferase 1\nCalculated Molecular Weight: 45 kDa\nObserved Molecular Weight: 38-45 kDa\nGenBank Accession Number: BC010942\nGene Symbol: ACAT1\nGene ID (NCBI): 38\nRRID: AB_2220210\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: P24752\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868849393833,"sku":"16215-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-16215-1-ap","provider":"Iright","version":"1.0","type":"link"}