{"product_id":"proteintech-16229-1-ap","title":"Proteintech, 16229-1-AP, HEXB Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe HEXB (16229-1-AP) by Proteintech is a Polyclonal antibody targeting HEXB in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples\n16229-1-AP targets HEXB in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HEK-293 cells, mouse kidney tissue,  Jurkat cells,  HeLa cells,  mouse lung tissue,  rat kidney tissue\nPositive IHC detected in: human kidney tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:2000\nImmunohistochemistry (IHC): IHC : 1:20-1:200\n\u003cb\u003eBackground Information\u003c\/b\u003e\nThe HEXB (Beta-hexosaminidase subunit beta) gene encodes the beta subunit of the enzyme hexosaminidase, which is involved in the breakdown of gangliosides. It is responsible for the degradation of GM2 gangliosides, and has a variety of other molecules containing terminal N-acetyl hexosamines, in the brain and other tissues. Refer to the domains of HEXB and glycosylation, there will be some different bands with MW 63-67 kDa precursor; 59-63 kDa signal peptide removed; 50-55 kDa subunit beta (or signal and propeptide removed); 20-25 kDa subunit beta chain B; ~30 kDa subunit beta chain A (Uniprot).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, mouse\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag8683 Product name: Recombinant human HEXB protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-332 aa of BC017378 Sequence: MELCGLGLPRPPMLLALLLATLLAAMLALLTQVALVVQVAEAARAPSVSAKPGPALWPLPLSVKMTPNLLHLAPENFYISHSPNSTAGPSCTLLEEAFRRYHGYIFGFYKWHHEPAEFQAKTQVQQLLVSITLQSECDAFPNISSDESYTLLVKEPVAVLKANRVWGALRGLETFSQLVYQDSYGTFTINESTIIDSPRFSHRGILIDTSRHYLPVKIILKTLDAMAFNKFNVLHWHIVDDQSFPYQSITFPELSNKGSYSLSHVYTPNDVRMVIEYARLRGIRVLPEFDTPGHTLSWGKGQKDLLTPCYSRQNKLDSFGPINPTLNTTYSF Predict reactive species\nFull Name: hexosaminidase B (beta polypeptide)\nCalculated Molecular Weight: 556 aa, 63 kDa\nObserved Molecular Weight: 63-67 kDa\nGenBank Accession Number: BC017378\nGene Symbol: HEXB\nGene ID (NCBI): 3074\nRRID: AB_2264075\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: P07686\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868955496617,"sku":"16229-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-16229-1-ap","provider":"Iright","version":"1.0","type":"link"}