{"product_id":"proteintech-16314-1-ap","title":"Proteintech, 16314-1-AP, dGK Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe dGK (16314-1-AP) by Proteintech is a Polyclonal antibody targeting dGK in WB, IHC, ELISA applications with reactivity to human, mouse samples\n16314-1-AP targets dGK in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: mouse kidney tissue\nPositive IHC detected in: human hepatocirrhosis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:1000\nImmunohistochemistry (IHC): IHC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nDeoxyguanosine kinase (dGK) deficiency is a frequent cause of mitochondrial DNA depletion associated with a hepatocerebral phenotype. dGK is a member of the deoxyribonucleoside kinase family involved in phosphorylation of deoxyribonucleosides. In mammals, there are four deoxyribonucleoside kinases with overlapping specificities: two cytoplasmic enzymes, thymidine kinase 1 (TK1) and deoxycytidine kinase (dCK), and two mitochondrial enzymes, thymidine kinase 2 (TK2) and deoxyguanosine kinase (dGK). The human dGK protein is highly specific for purine substrates. Human dGK is a dimer of two identical subunits of 30 kDa. The three-dimensional structure of the human dGK monomer has been reported, providing an explanation for the substrate specificity of the enzyme. (PMID: 29786478, PMID: 11427893, PMID: 17073823)\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag9361 Product name: Recombinant human DGUOK protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-277 aa of BC015757 Sequence: MAAGRLFLSRLRAPFSSMAKSPLEGVSSSRGLHAGRGPRRLSIEGNIAVGKSTFVKLLTKTYPEWHVATEPVATWQNIQAAGTQKACTAQSLGNLLDMMYREPARWSYTFQTFSFLSRLKVQLEPFPEKLLQARKPVQIFERSVYSDRYIFAKNLFENGSLSDIEWHIYQDWHSFLLWEFASRITLHGFIYLQASPQVCLKRLYQRAREEEKGIELAYLEQLHGQHEAWLIHKTTKLHFEALMNIPVLVLDVNDDFSEEVTKQEDLMREVNTFVKNL Predict reactive species\nFull Name: deoxyguanosine kinase\nCalculated Molecular Weight: 277 aa, 32 kDa\nObserved Molecular Weight: 28-32 kDa, 56-64 kDa\nGenBank Accession Number: BC015757\nGene Symbol: DGUOK\nGene ID (NCBI): 1716\nRRID: AB_10638311\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q16854\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46887186137257,"sku":"16314-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-16314-1-ap","provider":"Iright","version":"1.0","type":"link"}