{"product_id":"proteintech-16478-1-ap","title":"Proteintech, 16478-1-AP, MVP\/LRP Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe MVP\/LRP (16478-1-AP) by Proteintech is a Polyclonal antibody targeting MVP\/LRP in WB, IHC, IF\/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples\n16478-1-AP targets MVP\/LRP in WB, IHC, IF\/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: A549 cells, RAW264.7,  human brain tissue,  mouse lung tissue,  rat lung tissue,  SH-SY5Y cells\nPositive IP detected in: mouse lung tissue\nPositive IHC detected in: human breast cancer tissue, human testis tissue,  human skin tissue,  human lung cancer tissue,  human kidney tissue,  human normal colonNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: HepG2 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:2000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:50-1:500\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nVaults are the largest cellular ribonuclear protein complexes with a hollow barrel-like structure, and have been associated with the MDR phenotype. Major vault protein (MVP) is the main component of vaults and is presumed to be involved in MDR. MVP is identical with the human lung resistance protein (LRP), known to be overexpressed in multiple chemotherapy resistance models [PMID:23083532]. MVP has been associated to resistance to radiotherapy [4], probably due to its role in preventing apoptosis by inhibiting the COP-1\/p53 axis [PMID: 12894554, 15994960].\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, mouse\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag9593 Product name: Recombinant human MVP protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 544-893 aa of BC015623 Sequence: NWHFEVNDRKDPQETAKLFSVPDFVGDACKAIASRVRGAVASVTFDDFHKNSARIIRTAVFGFETSEAKGPDGMALPRPRDQAVFPQNGLVVSSVDVQSVEPVDQRTRDALQRSVQLAIEITTNSQEAAAKHEAQRLEQEARGRLERQKILDQSEAEKARKELLELEALSMAVESTGTAKAEAESRAEAARIEGEGSVLQAKLKAQALAIETEAELQRVQKVRELELVYARAQLELEVSKAQQLAEVEVKKFKQMTEAIGPSTIRDLAVAGPEMQVKLLQSLGLKSTLITDGSTPINLFNTAFGLLGMGPEGQPLGRRVASGPSPGEGISPQSAQAPQAPGDNHVVPVLR Predict reactive species\nFull Name: major vault protein\nCalculated Molecular Weight: 893 aa, 99 kDa\nObserved Molecular Weight: 99-104 kDa\nGenBank Accession Number: BC015623\nGene Symbol: LRP\nGene ID (NCBI): 9961\nRRID: AB_2147597\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q14764\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868894253225,"sku":"16478-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-16478-1-ap","provider":"Iright","version":"1.0","type":"link"}