{"product_id":"proteintech-16605-1-ap","title":"Proteintech, 16605-1-AP, OTUD7B Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe OTUD7B (16605-1-AP) by Proteintech is a Polyclonal antibody targeting OTUD7B in WB, IP, IHC, ELISA applications with reactivity to human, mouse, rat samples\n16605-1-AP targets OTUD7B in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: Jurkat cells\nPositive IP detected in: Jurkat cells\nPositive IHC detected in: human kidney tissue, human heart tissue,  mouse kidney tissue,  mouse heart tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:1000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nOUTD7B is a member of the A20 family of the deubiquitinating cysteine proteases, which has deubiquitinating activity that is directed towards 'Lys-48' or 'Lys-63'-linked polyubiquitin chains. It plays a key role in the negative feedback regulation of NF-κB signaling in response to multiple stimuli and has been described as central gatekeeper in inflammation and immunity. The calculated molecular weight of OTUD7B is 93 kDa, but the modified OTUD7B is about 100 kDa.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, mouse, rat\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag9788 Product name: Recombinant human OTUD7B protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-355 aa of BC020622 Sequence: MTLDMDAVLSDFVRSTGAEPGLARDLLEGKNWDVNAALSDFEQLRQVHAGNLPPSFSEGSGGSRTPEKGFSDREPTRPPRPILQRQDDIVQEKRLSRGISHASSSIVSLARSHVSSNGGGGGSNEHPLEMPICAFQLPDLTVYNEDFRSFIERDLIEQSMLVALEQAGRLNWWVSVDPTSQRLLPLATTGDGNCLLHAASLGMWGFHDRDLMLRKALYALMEKGVEKEALKRRWRWQQTQQNEESGLVYTEDEWQKEWNELIKLASSEPRMHLGTNGANCGGVESSEEPVYESLEEFHVFVLAHVLRRPIVVVADTMLRDSGGEAFAPIPFGGIYLPLEVPASQCHRSPLVLAYD Predict reactive species\nFull Name: OTU domain containing 7B\nCalculated Molecular Weight: 427aa,48 kDa; 843aa,93 kDa\nObserved Molecular Weight: 100 kDa\nGenBank Accession Number: BC020622\nGene Symbol: OTUD7B\nGene ID (NCBI): 56957\nRRID: AB_2157149\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q6GQQ9\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868868202665,"sku":"16605-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-16605-1-ap","provider":"Iright","version":"1.0","type":"link"}