{"product_id":"proteintech-16825-1-ap","title":"Proteintech, 16825-1-AP, GFAP Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe GFAP (16825-1-AP) by Proteintech is a Polyclonal antibody targeting GFAP in WB, IHC, IF\/ICC, IF-P, IF-Fro, ELISA applications with reactivity to human, mouse, rat samples\n16825-1-AP targets GFAP in WB, IHC, IF\/ICC, IF-P, IF-Fro, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: mouse brain tissue, U-251 cells,  rat brain tissue\nPositive IHC detected in: mouse brain tissue, Alzheimer mouse,  human brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF-P detected in: rat brain tissue, mouse brain tissue\nPositive IF-Fro detected in: mouse brain tissue\nPositive IF\/ICC detected in: A172 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:20000-1:100000\nImmunohistochemistry (IHC): IHC : 1:2500-1:10000\nImmunofluorescence (IF)-P: IF-P : 1:50-1:500\nImmunofluorescence (IF)-FRO: IF-FRO : 1:500-1:2000\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nFunctionGFAP (Glial fibrillary acidic protein) is a type III intermediate filament (IF) protein specific to the central \n                                        nervous system (CNS). GFAP is one of the  main components of the intermediate filament network in astrocytes and has\n                                         been proposed as playing a role in cell migration, cell motility, maintaining mechanical strength, and in mitosis.Tissue specificityGFAP is expressed in central nervous system cells, predominantly in astrocytes. GFAP is commonly used as an astrocyte marker. \n                                            However, GFAP is also present in peripheral glia and in non-CNS cells, including fibroblasts, chondrocytes, lymphocytes,\n                                             and liver stellate cells (PMID: 21219963).Involvement in diseaseMutations in GFAP lead to Alexander disease (OMIM: 203450), an autosomal dominant CNS disorder. The mutations\n                                                present in affected individuals are thought to be gain-of-function.Upregulation of GFAP is a hallmark of reactive astrocytes, in which GFAP is present in hypertrophic cellular\n                                                processes. Reactive astrogliosis is present in many neurological disorders, such as stroke, various\n                                                neurodegenerative diseases (including Alzheimer's and Parkinson's disease), and neurotrauma.IsoformsAstrocytes express 10 different isoforms of GFAP that differ in the rod and tail domains (PMID: 25726916), \n                                            which means that they differ in molecular size. Isoform expression varies\n                                             during the development and across different subtypes of astrocytes. Not all isoforms are upregulated in reactive astrocytes.Post-translational modificationsIntermediate filament proteins are regulated by phosphorylation. Six phosphorylation sites have been identified in GFAP\n                                             protein, at least some of which are reported to control filament assembly (PMID: 21219963).Cellular localizationGFAP localizes to intermediate filaments and stains well in astrocyte cellular processes.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, mouse, rat, pig, rabbit, monkey, zebrafish, hamster, goat, duck\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag10423 Product name: Recombinant human GFAP protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 83-432 aa of BC013596 Sequence: YIEKVRFLEQQNKALAAELNQLRAKEPTKLADVYQAELRELRLRLDQLTANSARLEVERDNLAQDLATVRQKLQDETNLRLEAENNLAAYRQEADEATLARLDLERKIESLEEEIRFLRKIHEEEVRELQEQLARQQVHVELDVAKPDLTAALKEIRTQYEAMASSNMHEAEEWYRSKFADLTDAAARNAELLRQAKHEANDYRRQLQSLTCDLESLRGTNESLERQMREQEERHVREAASYQEALARLEEEGQSLKDEMARHLQEYQDLLNVKLALDIEIATYRKLLEGEENRITIPVQTFSNLQIRETSLDTKSVSEGHLKRNIVVKTVEMRDGEVIKESKQEHKDVM Predict reactive species\nFull Name: glial fibrillary acidic protein\nCalculated Molecular Weight: 432 aa, 50 kDa\nObserved Molecular Weight: 45-50 kDa\nGenBank Accession Number: BC013596\nGene Symbol: GFAP\nGene ID (NCBI): 2670\nRRID: AB_2109646\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: P14136\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868818428073,"sku":"16825-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-16825-1-ap","provider":"Iright","version":"1.0","type":"link"}