{"product_id":"proteintech-17034-1-ap","title":"Proteintech, 17034-1-AP, Methylmalonyl Coenzyme A mutase\/MUT Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe Methylmalonyl Coenzyme A mutase\/MUT (17034-1-AP) by Proteintech is a Polyclonal antibody targeting Methylmalonyl Coenzyme A mutase\/MUT in WB, IHC, IP, ELISA applications with reactivity to human, mouse, rat samples\n17034-1-AP targets Methylmalonyl Coenzyme A mutase\/MUT in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HEK-293 cells, HeLa cells,  HepG2 cells\nPositive IP detected in: HepG2 cells\nPositive IHC detected in: human liver cancer tissue, human prostate cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:2000-1:12000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nMethylmalonyl Coenzyme A mutase (MUT) is an enzyme that plays a crucial role in the metabolism of certain amino acids and fatty acids (PMID: 15647267). Mutations in the MUT gene can lead to methylmalonic acidemia, a metabolic disorder characterized by the accumulation of toxic compounds such as methylmalonyl-CoA and propionyl-CoA (PMID: 30428564), resulting in life-threatening metabolic acidosis, respiratory distress, neurological impairment, hyperammonemia, and ketosis (PMID: 32679819). MUT is essential for maintaining normal metabolic processes and its dysfunction can have significant health implications, highlighting its importance in both basic metabolism and clinical medicine (PMID: 23041189).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, mouse\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag10523 Product name: Recombinant human MUT protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 401-750 aa of BC016282 Sequence: SARIARNTQIIIQEESGIPKVADPWGGSYMMECLTNDVYDAALKLINEIEEMGGMAKAVAEGIPKLRIEECAARRQARIDSGSEVIVGVNKYQLEKEDTVEVLAIDNTSVRNRQIEKLKKIKSSRDQALAERCLAALTECAASGDGNILALAVDASRARCTVGEITDALKKVFGEHKANDRMVSGAYRQEFGESKEITSAIKRVHKFMEREGRRPRLLVAKMGQDGHDRGAKVIATGFADLGFDVDIGPLFQTPREVAQQAVDADVHAVGVSTLAAGHKTLVPELIKELNSLGRPDILVMCGGVIPPQDYEFLFEVGVSNVFGPGTRIPKAAVQVLDDIEKCLEKKQQSV Predict reactive species\nFull Name: methylmalonyl Coenzyme A mutase\nCalculated Molecular Weight: 750 aa, 83 kDa\nObserved Molecular Weight: 78 kDa\nGenBank Accession Number: BC016282\nGene Symbol: MUT\nGene ID (NCBI): 4594\nRRID: AB_2147263\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: P22033\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868817346729,"sku":"17034-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-17034-1-ap","provider":"Iright","version":"1.0","type":"link"}