{"product_id":"proteintech-17699-1-ap","title":"Proteintech, 17699-1-AP, ICAM3\/CD50 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe ICAM3\/CD50 (17699-1-AP) by Proteintech is a Polyclonal antibody targeting ICAM3\/CD50 in WB, IHC, IP, ELISA applications with reactivity to human samples\n17699-1-AP targets ICAM3\/CD50 in WB, IHC, IP, ELISA applications and shows reactivity with human samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: Jurkat cells, U-937 cells,  Ramos cells\nPositive IP detected in: Jurkat cells\nPositive IHC detected in: human colon tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:2400\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:20-1:200\n\u003cb\u003eBackground Information\u003c\/b\u003e\nICAM3 (Intercellular adhesion molecule 3) is also named as CD50, CDw50 and ICAM-R. ICAM3 is a member of the intercellular adhesion molecule (ICAM) family. ICAM3, being expressed constitutively on T cells and other leukocytes, is a potent signalling molecule and a major ligand in the initiation of T cell-mediated immune responses (PMID: 1448173, PMID: 11812993). ICAM3 is the natural ligand for both DC-SIGN and L-SIGN (PMID: 17570115). ICAM3 is abundantly and constitutively expressed in all leukocytes and is probably the most important ligand for LFA-1 in initiating immune responses, and ICAM3 is a typical type I transmembrane glycoprotein (PMID: 39991572). The promoter region of ICAM3 can be bound by co-transcription factors such as ETs and C\/EBP,  promoting macrophage maturation as well as monocyte extravasation (PMID: 22479382). ICAM3 is widely expressed not only in lymphocytes such as T and B cells but also in many endothelial cells and dendritic cells (PMID: 39991572).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag11955 Product name: Recombinant human ICAM3 protein Source: e coli. -derived, T-HIS Tag: 6*His Domain: 200-547 aa of BC058903 Sequence: GLGLFVNTSAPRQLRTFVLPVTPPRLVAPRFLEVETSWPVDCTLDGLFPASEAQVYLALGDQMLNATVMNHGDTLTATATATARADQEGAREIVCNVTLGGERREARENLTVFSFLGPIVNLSEPTAHEGSTVTVSCMAGARVQVTLDGVPAAAPGQPAQLQLNATESDDGRSFFCSATLEVDGEFLHRNSSVQLRVLYGPKIDRATCPQHLKWKDKTRHVLQCQARGNPYPELRCLKEGSSREVPVGIPFFVNVTHNGTYQCQASSSRGKYTLVVVMDIEAGSSHFVPVFVAVLLTLGVVTIVLALMYVFREHQRSGSYHVREESTYLPLTSMQPTEAMGEEPSRAE Predict reactive species\nFull Name: intercellular adhesion molecule 3\nCalculated Molecular Weight: 547 aa, 60 kDa\nObserved Molecular Weight: 110-160 kDa, 60 kDa\nGenBank Accession Number: BC058903\nGene Symbol: ICAM3\/CD50\nGene ID (NCBI): 3385\nRRID: AB_2248763\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: P32942\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46887674904745,"sku":"17699-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-17699-1-ap","provider":"Iright","version":"1.0","type":"link"}