{"product_id":"proteintech-17711-1-ap","title":"Proteintech, 17711-1-AP, ARL13B Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe ARL13B (17711-1-AP) by Proteintech is a Polyclonal antibody targeting ARL13B in WB, IHC, IF\/ICC, IP, ELISA applications with reactivity to human, mouse, rat, canine samples\n17711-1-AP targets ARL13B in WB, IHC, IF\/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat, canine samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: L02 cells, mouse brain tissue,  mouse kidney tissue,  mouse liver tissue,  NIH\/3T3 cells,  rat liver tissue,  rat kidney tissue\nPositive IP detected in: L02 cells\nPositive IHC detected in: mouse brain tissue, human liver tissue,  human testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: hTERT-RPE1 cells, NIH3T3 cells,  MDCK cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:1000-1:6000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:50-1:500\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:200-1:800\n\u003cb\u003eBackground Information\u003c\/b\u003e\nARL13B, also named ARL2L1, is the Ras superfamily's small ciliary G protein. Localized in the cilia, it is required for cilium biogenesis and sonic hedgehog signaling. Defects in ARL13B cause Joubert syndrome (JS), an autosomal recessive disorder characterized by a distinctive cerebellar malformation (PMID: 19906870). This antibody detects two specific bands at 60 kDa and 48 kDa. Arl13b is predicted to be a 48 kDa protein, and the 60 kDa band will likely represent a modified form of Arl13b. ARL13B can be used to mark the cilia (PMID:22072986).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat, canine\nCited Reactivity: human, mouse, rat, pig, canine, monkey, chicken, zebrafish, sheep, xenopus\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag12015 Product name: Recombinant human ARL13B protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-321 aa of BC094725 Sequence: MFSLMASCCGWFKRWREPVRLANKQDKEGALGEADVIECLSLEKLVNEHKCLCQIEPCSAISGYGKKIDKSIKKGLYWLLHVIARDFDALNERIQKETTEQRALEEQEKQERAERVRKLREERKQNEQEQAELDGTSGLAELDPEPTNPFQPIASVIIENEGKLEREKKNQKMEKDSDGCHLKHKMEHEQIETQGQVNHNGQKNNEFGLVENYKEALTQQLKNEDETDRPSLESANGKKKTKKLRMKRNHRVEPLNIDDCAPESPTPPPPPPPVGWGTPKVTRLPKLEPLGETHHNDFYRKPLPPLAVPQRPNSDAHDVIS Predict reactive species\nFull Name: ADP-ribosylation factor-like 13B\nCalculated Molecular Weight: 48 kDa\nObserved Molecular Weight: 40-48 kDa, 60 kDa\nGenBank Accession Number: BC094725\nGene Symbol: ARL13B\nGene ID (NCBI): 200894\nRRID: AB_2060867\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q3SXY8\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868817871017,"sku":"17711-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-17711-1-ap","provider":"Iright","version":"1.0","type":"link"}