{"product_id":"proteintech-17950-1-ap","title":"Proteintech, 17950-1-AP, GPR177 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe GPR177 (17950-1-AP) by Proteintech is a Polyclonal antibody targeting GPR177 in WB, IP, IHC, ELISA applications with reactivity to human,  mouse,  rat samples\n17950-1-AP targets GPR177 in WB, IHC, IP, CoIP, ELISA applications and shows reactivity with human,  mouse,  rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: U-87 MG cells, mouse brain tissue,  HEK-293 cells,  rat brain tissue\nPositive IP detected in: mouse liver tissue\nPositive IHC detected in: human ovary tissue, mouse liver tissue,  human kidney tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:1000-1:5000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nGPR177, also known as Wntless (WLS) or Evi, is an evolutionarily conserved transmembrane protein necessary for Wnt protein secretion. GPR177 localizes to compartments of the secretory pathway, including the ER, Golgi apparatus, endosomes, and plasma membrane. GPR177 is overexpressed in several cancer types including glioma and gastric cancer. Several isoforms of GPR177 exist due to the alternative splicing, with predicted MW around 62 and 51 kDa, respectively. The sequence can be processed by removing the signal peptide, which generates a mature form of 45-46 kDa. In addition, slow migragrating band above 62 kDa can also be detected because GPR177 is modified by N-linked glycosylation (PMID: 20549736).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, mouse, rat, goat\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag12428 Product name: Recombinant human GPR177 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 34-239 aa of BC110826 Sequence: LIAPGPTTAVSYMSVKCVDARKNHHKTKWFVPWGPNHCDKIRDIEEAIPREIEANDIVFSVHIPLPHMEMSPWFQFMLFILQLDIAFKLNNQIRENAEVSMDVSLAYRDDAFAEWTEMAHERVPRKLKCTFTSPKTPEHEGRYYECDVLPFMEIGSVAHKFYLLNIRLPVNEKKKINVGIGEIKDIRLVGIHQNGGFTKVWFAMKT Predict reactive species\nFull Name: G protein-coupled receptor 177\nCalculated Molecular Weight: 541 aa, 62 kDa\nObserved Molecular Weight: 46-65 kDa\nGenBank Accession Number: BC110826\nGene Symbol: GPR177\nGene ID (NCBI): 79971\nRRID: AB_2034928\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q5T9L3\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868941439145,"sku":"17950-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-17950-1-ap","provider":"Iright","version":"1.0","type":"link"}