{"product_id":"proteintech-18065-1-ap","title":"Proteintech, 18065-1-AP, ALDOB Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe ALDOB (18065-1-AP) by Proteintech is a Polyclonal antibody targeting ALDOB in WB, IHC, IP, ELISA applications with reactivity to human, mouse, rat samples\n18065-1-AP targets ALDOB in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: mouse kidney tissue, mouse liver tissue,  mouse brain tissue,  Raji cells,  mouse skeletal muscle tissue,  rat kidney tissue\nPositive IP detected in: mouse brain tissue, mouse kidney tissue\nPositive IHC detected in: human liver cancer tissue, human liver tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:2000-1:16000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nFructose-1,6-bisphosphate aldolase is a glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate.Vertebrates have 3 aldolase isozymes, aldolase A (ALDOA), B (ALDOB), and C (ALDOC). Deficiency of this enzyme ALDOB causes an accumulation of fructose-1-phosphate after fructose intake, which results in toxic symptoms like vomiting, hypoglycemia, jaundice, elevated liver enzymes and hepatomegaly(PMID:22375183). This antibody may detect ALDOA.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, mouse, rat\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag12695 Product name: Recombinant human ALDOB protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-316 aa of BC029399 Sequence: MAHRFPALTQEQKKELSEIAQSIVANGKGILAADESVGTMGNRLQRIKVENTEENRRQFREILFSVDSSINQSIGGVILFHETLYQKDSQGKLFRNILKEKGIVVGIKLDQGGAPLAGTNKETTIQGLDGLSERCAQYKKDGVDFGKWRAVLRIADQCPSSLPIQENANALARYASICQQNGLVPIVEPEVIPDGDHDLEHCQYVTEKVLAAVYKALNDHHVYLEGTLLKPNMVTAGHACTKKYTPEQVAMATVTALHRTVPAAVPGICFLSGGMSEEDATLNLNAINLCPLPKPWKLSFSGVARLQTRRQPRRLL Predict reactive species\nFull Name: aldolase B, fructose-bisphosphate\nCalculated Molecular Weight: 316 aa, 35 kDa\nObserved Molecular Weight: 35-40 kDa\nGenBank Accession Number: BC029399\nGene Symbol: ALDOB\nGene ID (NCBI): 229\nRRID: AB_2273968\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: P05062\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868852342953,"sku":"18065-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-18065-1-ap","provider":"Iright","version":"1.0","type":"link"}