{"product_id":"proteintech-18166-1-ap","title":"Proteintech, 18166-1-AP, WISP-1\/CCN4 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe WISP-1\/CCN4 (18166-1-AP) by Proteintech is a Polyclonal antibody targeting WISP-1\/CCN4 in WB, IHC, ELISA applications with reactivity to human, mouse samples\n18166-1-AP targets WISP-1\/CCN4 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: COLO 320 cells, HEK-293 cells,  mouse testis tissue,  mouse liver tissue\nPositive IHC detected in: human stomach cancer tissue, human small intestine tissue,  human lung cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:1000-1:4000\nImmunohistochemistry (IHC): IHC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nWISP1( Wnt-induced secreted protein 1), also known as CCN4, is a secreted, matricellular protein allocated to the CCN protein family. The CCN protein family consists of six, modular structured, secreted proteins ( Cyr61, CTGF, Nov, WISP1, WISP2, WISP3 ). WISP1 may be downstream in the WNT1 signaling pathway that is relevant to malignant transformation. WISP1 is expressed at a high level in fibroblast cells, and overexpressed in colon tumors. Its expression is associated with proliferation, cytoprotection, as well as extracellular matrix production. 18166-1-AP antibody detects the 40-45 kDa protein in SDS-PAGE.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse\nCited Reactivity: human\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag12657 Product name: Recombinant human WISP1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 132-367 aa of BC074841 Sequence: NGQSFQPNCKYNCTCIDGAVGCTPLCLRVRPPRLWCPHPRRVSIPGHCCEQWVCEDDAKRPRKTAPRDTGAFDAVGEVEAWHRNCIAYTSPWSPCSTSCGLGVSTRISNVNAQCWPEQESRLCNLRPCDVDIHTLIKAGKKCLAVYQPEASMNFTLAGCISTRSYQPKYCGVCMDNRCCIPYKSKTIDVSFQCPDGLGFSRQVLWINACFCNLSCRNPNDIFADLESYPDFSEIAN Predict reactive species\nFull Name: WNT1 inducible signaling pathway protein 1\nCalculated Molecular Weight: 367 aa, 40 kDa\nObserved Molecular Weight: 40-45 kDa\nGenBank Accession Number: BC074841\nGene Symbol: WISP1\nGene ID (NCBI): 8840\nRRID: AB_10805764\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: O95388\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868946616489,"sku":"18166-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-18166-1-ap","provider":"Iright","version":"1.0","type":"link"}