{"product_id":"proteintech-18227-1-ap","title":"Proteintech, 18227-1-AP, CYP3A4 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe CYP3A4 (18227-1-AP) by Proteintech is a Polyclonal antibody targeting CYP3A4 in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples\n18227-1-AP targets CYP3A4 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: rat liver tissue, mouse liver tissue,  mouse brain tissue\nPositive IHC detected in: human liver tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:1000-1:4000\nImmunohistochemistry (IHC): IHC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nCytochrome P450 3A(CYP3A) belongs to the cytochrome P450 family and the enzymes constitute an important detoxification system that contributes to primary metabolism of more than half of all prescribed medications.CYP3A expression determines impairment of drug absorption and efficient systemic clearance in a tissue-specific manner(PMID:17975676). The highest amounts of drug-metabolizing cytochromes P-450 are found in the liver and intestine, with lower amounts found in other organs, including the kidney, lung, and brain. LPS that has reached the circulation stimulates a TLR4-dependent mechanism in the periphery, evoking a reduction in Cyp3a11 expression and metabolism in the liver (PMID: 15878984). Human CYP3A4 is homologous to five mouse P450 isoforms, namely, Cyp3a11, Cyp3a16, Cyp3a41a, Cyp3a41b, and Cyp3a44 (PMID: 22446519). And CYP3A4 is also the human homologue of rat CYP3A1 and CYP3A2 (PMID: 27546062, PMID: 32874920).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, mouse, rat, monkey\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag13074 Product name: Recombinant human CYP3A4 protein Source: e coli. -derived, T-HIS Tag: 6*His Domain: 154-503 aa of BC101631 Sequence: DVLVRNLRREAETGKPVTLKDVFGAYSMDVITSTSFGVNIDSLNNPQDPFVENTKKLLRFDFLDPFFLSITVFPFLIPILEVLNICVFPREVTNFLRKSVKRMKESRLEDTQKHRVDFLQLMIDSQNSKETESHKALSDLELVAQSIIFIFAGYETTSSVLSFIMYELATHPDVQQKLQEEIDAVLPNKAPPTYDTVLQMEYLDMVVNETLRLFPIAMRLERVCKKDVEINGMFIPKGVVVMIPSYALHRDPKYWTEPEKFLPERFSKKNKDNIDPYIYTPFGSGPRNCIGMRFALMNMKLALIRVLQNFSFKPCKETQIPLKLSLGGLLQPEKPVVLKVESRDGTVSGA Predict reactive species\nFull Name: cytochrome P450, family 3, subfamily A, polypeptide 4\nCalculated Molecular Weight: 503 aa, 57 kDa\nObserved Molecular Weight: 51-57 kDa\nGenBank Accession Number: BC101631\nGene Symbol: CYP3A4\nGene ID (NCBI): 1576\nRRID: AB_2090329\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: P08684\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868834484393,"sku":"18227-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-18227-1-ap","provider":"Iright","version":"1.0","type":"link"}