{"product_id":"proteintech-18301-1-ap","title":"Proteintech, 18301-1-AP, COL18A1 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe COL18A1 (18301-1-AP) by Proteintech is a Polyclonal antibody targeting COL18A1 in WB, IHC, IF, IF-P, ELISA applications with reactivity to human, mouse, zebrafish samples\n18301-1-AP targets COL18A1 in WB, IHC, IF, IF-P, ELISA applications and shows reactivity with human, mouse, zebrafish samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: human kidney tissue\nPositive IHC detected in: human liver tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF-P detected in: mouse eye tissue\nPositive IF detected in: zebrafish retina\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:200-1:1000\nImmunohistochemistry (IHC): IHC : 1:20-1:200\nImmunofluorescence (IF)-P: IF-P : 1:50-1:500\nImmunofluorescence (IF): IF : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nCOL18A1 is a naturally-occurring 20-kDa C-terminal fragment derived from collagen alpha-1(XVIII) chain. It is reported to serve as an anti-angiogenic agent, similar to angiostatin and thrombospondin. It potently inhibits endothelial cell proliferation and inhibits endothelial cell angiogenesis by binding to the heparan sulfate proteoglycans involved in growth factor signaling. COL18A1 is a broad spectrum angiogenesis inhibitor and may interfere with the pro-angiogenic action of growth factors such as basic fibroblast growth factor (bFGF\/FGF-2) and vascular endothelial growth factor (VEGF). This antibody raised against 1693-1706 aa of human collagen alpha-1(XVIII) chain can recognize endostatin (20 kDa) and several endostatin-related fragments of higher molecular weight (PMID: 15545970; 19358707).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, zebrafish\nCited Reactivity: human, rat\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: collagen, type XVIII, alpha 1\nCalculated Molecular Weight: 178 kDa\nObserved Molecular Weight: 20 kDa\nGenBank Accession Number: BC063833\nGene Symbol: COL18A1\/Endostatin\nGene ID (NCBI): 80781\nRRID: AB_2081719\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: P39060\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868995834025,"sku":"18301-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-18301-1-ap","provider":"Iright","version":"1.0","type":"link"}