{"product_id":"proteintech-18350-1-ap","title":"Proteintech, 18350-1-AP, DTX1 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe DTX1 (18350-1-AP) by Proteintech is a Polyclonal antibody targeting DTX1 in WB, IHC, IF, ELISA applications with reactivity to human, mouse, rat samples\n18350-1-AP targets DTX1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: Daudi cells, A375 cells\nPositive IHC detected in: human colon tissue, human gliomas tissue,  human testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF detected in: mouse olfactory epithelium tissue\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:1000\nImmunohistochemistry (IHC): IHC : 1:150-1:600\nImmunofluorescence (IF): IF : 1:50-1:200\n\u003cb\u003eBackground Information\u003c\/b\u003e\nDeltex homolog 1 (E3 ubiquitin-protein ligase DTX1) is a regulator of Notch signaling, a signaling pathway involved in cell-cell communications that regulates a broad spectrum of cell-fate determinations. Probably acts both as a positive and negative regulator of Notch, depending on the developmental and cell context. Functions as an ubiquitin ligase protein in vivo, mediating ubiquitination and promoting degradation of MEKK1, suggesting that it may regulate the Notch pathway via some ubiquitin ligase activity.DTX1 is conserved from Drosophila, and is expressed in a wide array of tissues, particularly in the central nervous system, testes, and endothelial cells, mainly colocalized with Notch1.It contains a RING-type zinc finger and 2 WWE domains.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag13172 Product name: Recombinant human DTX1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-374 aa of BC048216 Sequence: MSRPGHGGLMPVNGLGFPPQNVARVVVWEWLNEHSRWRPYTATVCHHIENVLKEDARGSVVLGQVDAQLVPYIIDLQSMHQFRQDTGTMRPVRRNFYDPSSAPGKGIVWEWENDGGAWTAYDMDICITIQNAYEKQHPWLDLSSLGFCYLIYFNSMSQMNRQTRRRRRLRRRLDLAYPLTVGSIPKSQSWPVGASSGQPCSCQQCLLVNSTRAASNAILASQRRKAPPAPPLPPPPPPGGPPGALAVRPSATFTGAALWAAPAAGPAEPAPPPGAPPRSPGAPGGARTPGQNNLNRPGPQRTTSVSARASIPPGVPALPVKNLNGTGPVHPALAGMTGILLCAAGLPVCLTRAPKPILHPPPVSKSDVKPVPGV Predict reactive species\nFull Name: deltex homolog 1 (Drosophila)\nCalculated Molecular Weight: 620 aa, 67 kDa\nObserved Molecular Weight: 67 kDa\nGenBank Accession Number: BC048216\nGene Symbol: DTX1\nGene ID (NCBI): 1840\nRRID: AB_2878536\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q86Y01\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46869532967081,"sku":"18350-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-18350-1-ap","provider":"Iright","version":"1.0","type":"link"}