{"product_id":"proteintech-18355-1-ap","title":"Proteintech, 18355-1-AP, PLD1 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe PLD1 (18355-1-AP) by Proteintech is a Polyclonal antibody targeting PLD1 in WB, IHC, IF\/ICC, ELISA applications with reactivity to human, mouse samples\n18355-1-AP targets PLD1 in WB, IHC, IF\/ICC, ELISA applications and shows reactivity with human, mouse samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HeLa cells\nPositive IHC detected in: mouse heart tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: HeLa cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:1000-1:4000\nImmunohistochemistry (IHC): IHC : 1:50-1:500\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nPLD1(Phospholipase D1) belongs to the phospholipase D family. It is implicated in the release process of a variety of hormones, most likely at a late stage of exocytosis through the production of lipids that are essential for the fusion process and seem to be also essential for fast neurotransmitter release. PLD1 is primarily localized in vesicular structures such as endosomes, lysosomes, and autophagosomes. PLD1 has four alternative splicing variants: PLD1a (124 kDa), PLD1b (119 kDa), PLD1c (69 kDa), PLD1d (112 kDa) (PMID: 18636075).  It is best to use unboiled samples for detection, for membrane proteins are hydrophobic and tend to aggregate when boiled at high temperatures.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse\nCited Reactivity: human\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag13227 Product name: Recombinant human PLD1 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 725-1074 aa of BC068976 Sequence: TTAHELRYQVPGSVHANVQLLRSAADWSAGIKYHEESIHAAYVHVIENSRHYIYIENQFFISCADDKVVFNKIGDAIAQRILKAHRENQKYRVYVVIPLLPGFEGDISTGGGNALQAIMHFNYRTMCRGENSILGQLKAELGNQWINYISFCGLRTHAELEGNLVTELIYVHSKLLIADDNTVIIGSANINDRSMLGKRDSEMAVIVQDTETVPSVMDGKEYQAGRFARGLRLQCFRVVLGYLDDPSEDIQDPVSDKFFKEVWVSTAARNATIYDKVFRCLPNDEVHNLIQLRDFINKPVLAKEDPIRAEEELKKIRGFLVQFPFYFLSEESLLPSVGTKEAIVPMEVWT Predict reactive species\nFull Name: phospholipase D1, phosphatidylcholine-specific\nCalculated Molecular Weight: 1074 aa, 124 kDa\nObserved Molecular Weight: 112-124 kDa\nGenBank Accession Number: BC068976\nGene Symbol: PLD1\nGene ID (NCBI): 5337\nRRID: AB_2163859\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q13393\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46869731279017,"sku":"18355-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-18355-1-ap","provider":"Iright","version":"1.0","type":"link"}