{"product_id":"proteintech-18443-1-ap","title":"Proteintech, 18443-1-AP, UQCRFS1 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe UQCRFS1 (18443-1-AP) by Proteintech is a Polyclonal antibody targeting UQCRFS1 in WB, IHC, IF\/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples\n18443-1-AP targets UQCRFS1 in WB, IHC, IF\/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: MCF-7 cells, mouse heart tissue,  rat heart tissue\nPositive IP detected in: mouse heart tissue\nPositive IHC detected in: human stomach cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: MCF-7 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:1000-1:8000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:250-1:1000\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nThe ubiquinol cytochrome c reductase UQCRFS1 is a key subunit of the cytochrome bc1 complex (complex III) of the mitochondrial respiratory chain. The UQCRFS1 gene encode a 30 kDa protein with a transit peptide and it appears to be involved in development of more aggressive phenotype of breast cancer(PMID:15047214). UQCRFS1 is also named as RISP, complex III subunit 5, cytochrome b-c1 complex subunit 5 and rieske iron-sulfur protein. This antibody is specific to UQCRFS1.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, mouse, rat, pig\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag13315 Product name: Recombinant human UQCRFS1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-274 aa of BC010035 Sequence: MLSVAARSGPFAPVLSATSRGVAGALRPLVQATVPATPEQPVLDLKRPFLSRESLSGQAVRRPLVASVGLNVPASVCYSHTDIKVPDFSEYRRLEVLDSTKSSRESSEARKGFSYLVTGVTTVGVAYAAKNAVTQFVSSMSASADVLALAKIEIKLSDIPEGKNMAFKWRGKPLFVRHRTQKEIEQEAAVELSQLRDPQHDLDRVKKPEWVILIGVCTHLGCVPIANAGDFGGYYCPCHGSHYDASGRIRLGPAPLNLEVPTYEFTSDDMVIVG Predict reactive species\nFull Name: ubiquinol-cytochrome c reductase, Rieske iron-sulfur polypeptide 1\nCalculated Molecular Weight: 30 kDa\nObserved Molecular Weight: 25 kDa\nGenBank Accession Number: BC010035\nGene Symbol: UQCRFS1\nGene ID (NCBI): 7386\nRRID: AB_10699875\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: P47985\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868856144041,"sku":"18443-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-18443-1-ap","provider":"Iright","version":"1.0","type":"link"}