{"product_id":"proteintech-19118-1-ap","title":"Proteintech, 19118-1-AP, RASSF4 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe RASSF4 (19118-1-AP) by Proteintech is a Polyclonal antibody targeting RASSF4 in WB, IHC, ELISA applications with reactivity to human samples\n19118-1-AP targets RASSF4 in WB, IHC, ELISA applications and shows reactivity with human samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HCT 116 cells, NCI-H1299 cells,  U2OS cells,  MG-63 cells\nPositive IHC detected in: mouse lung tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:2000\nImmunohistochemistry (IHC): IHC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nRASSF4, a member of the classical RASSF family of scaffold proteins, is associated with alveolar rhabdomyosarcoma (aRMS), an aggressive pediatric cancer of muscle histogenesis. In aRMS, RASSF4 is upregulated in human cell lines and tumors and is oncogenic, portending a worse clinical prognosis. RASSF4 has been reported to directly interact with and activate K‐Ras in a GTP‐dependent manner. Ectopic expression of RASSF4 induces 293T apoptosis in a Ras‐dependent manner and inhibits tumour cell growth.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human\nCited Reactivity: human\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag5780 Product name: Recombinant human RASSF4 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-321 aa of BC032593 Sequence: MKEDCLPSSHVPISDSKSIQKSELLGLLKTYNCYHEGKSFQLRHREEEGTLIIEGLLNIAWGLRRPIRLQMQDDREQVHLPSTSWMPRRPSCPLKEPSPQNGNITAQGPSIQPVHKAESSTDSSGPLEEAEEAPQLMRTKSDASCMSQRRPKCRAPGEAQRIRRHRFSINGHFYNHKTSVFTPAYGSVTNVRVNSTMTTLQVLTLLLNKFRVEDGPSEFALYIVHESGERTKLKDCEYPLISRILHGPCEKIARIFLMEADLGVEVPHEVAQYIKFEMPVLDSFVEKLKEEEEREIIKLTMKFQALRLTMLQRLEQLVEAK Predict reactive species\nFull Name: Ras association (RalGDS\/AF-6) domain family member 4\nCalculated Molecular Weight: 321 aa, 37 kDa\nObserved Molecular Weight: 34-37 kDa\nGenBank Accession Number: BC032593\nGene Symbol: RASSF4\nGene ID (NCBI): 83937\nRRID: AB_10596921\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q9H2L5\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46869581070505,"sku":"19118-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-19118-1-ap","provider":"Iright","version":"1.0","type":"link"}