{"product_id":"proteintech-19892-1-ap","title":"Proteintech, 19892-1-AP, ASUN Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe ASUN (19892-1-AP) by Proteintech is a Polyclonal antibody targeting ASUN in WB, IHC, IF\/ICC, IP, ELISA applications with reactivity to human samples\n19892-1-AP targets ASUN in WB, IHC, IF\/ICC, IP, ChIP, ELISA applications and shows reactivity with human samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HeLa cells, MCF-7 cells,  Jurkat cells\nPositive IP detected in: MCF-7 cells\nPositive IHC detected in: human cervical cancer tissue,  human testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: MCF-7 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:5000-1:50000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:20-1:200\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:200-1:800\n\u003cb\u003eBackground Information\u003c\/b\u003e\nAsunder (ASUN) is a conserved protein that has been reported as a critical regulator of dynein localization duringDrosophilaspermatogenesis (PMID: 19357193). Mammalian andDrosophilaASUN proteins revealed that they are 43% identical and 64% similar. Human ASUN (also known as GCT1 or C12ORF11) promotes dynein recruitment and centrosomal tethering to the nucleus at mitotic entry (PMID: 23097494).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human\nCited Reactivity: human\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag13727 Product name: Recombinant human C12orf11 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 357-706 aa of BC003081 Sequence: GRSVLLEQPRKSGSKVISHMLSSHGGEIFLHVLSSSRSILEDPPSISEGCGGRVTDYRITDFGEFMRENRLTPFLDPRYKIDGSLEVPLERAKDQLEKHTRYWPMIISQTTIFNMQAVVPLASVIVKESLTEEDVLNCQKTIYNLVDMERKNDPLPISTVGTRGKGPKRDEQYRIMWNELETLVRAHINNSEKHQRVLECLMACRSKPPEEEERKKRGRKREDKEDKSEKAVKDYEQEKSWQDSERLKGILERGKEELAEAEIIKDSPDSPEPPNKKPLVEMDETPQVEKSKGPVSLLSLWSNRINTANSRKHQEFAGRLNSVNNRAELYQHLKEENGMETTKNGKASRQ Predict reactive species\nFull Name: chromosome 12 open reading frame 11\nCalculated Molecular Weight: 706 aa, 80 kDa\nObserved Molecular Weight: 70-80 kDa\nGenBank Accession Number: BC003081\nGene Symbol: ASUN\nGene ID (NCBI): 55726\nRRID: AB_10638473\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q9NVM9\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46869393637545,"sku":"19892-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-19892-1-ap","provider":"Iright","version":"1.0","type":"link"}