{"product_id":"proteintech-19982-1-ap","title":"Proteintech, 19982-1-AP, PRKCQ-Specific Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe PRKCQ-Specific (19982-1-AP) by Proteintech is a Polyclonal antibody targeting PRKCQ-Specific in WB, FC (Intra), IP, ELISA applications with reactivity to human samples\n19982-1-AP targets PRKCQ-Specific in WB, FC (Intra), IP, ELISA applications and shows reactivity with human samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: Jurkat cells, MOLT-4 cells\nPositive IP detected in: K-562 cells\nPositive FC (Intra) detected in: K-562 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:1000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nFlow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension\n\u003cb\u003eBackground Information\u003c\/b\u003e\nPRKCQ, also named as PRKCT and nPKC-theta, belongs to the protein kinase superfamily, AGC Ser\/Thr protein kinase family and PKC subfamily. PRKCQ catalyzes the reaction: ATP + a protein = ADP + a phosphoprotein. PRKCQ is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. It is essential for T-cell receptor (TCR)-mediated T-cell activation, but it is dispensable during TCR-dependent thymocyte development. PRKCQ links the TCR signaling complex to the activation of NF-kappa-B in mature T lymphocytes. It is required for interleukin-2 (IL2) production. PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. This antibody is specific to PRKCQ.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: protein kinase C, theta\nCalculated Molecular Weight: 82 kDa\nObserved Molecular Weight: 72-74 kDa\nGenBank Accession Number: NM_006257\nGene Symbol: PRKCQ\nGene ID (NCBI): 5588\nRRID: AB_3669319\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q04759\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46887773536425,"sku":"19982-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-19982-1-ap","provider":"Iright","version":"1.0","type":"link"}