{"product_id":"proteintech-19990-1-ap","title":"Proteintech, 19990-1-AP, Hemoglobin Alpha-Specific Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe Hemoglobin Alpha-Specific (19990-1-AP) by Proteintech is a Polyclonal antibody targeting Hemoglobin Alpha-Specific in WB, IHC, ELISA applications with reactivity to human samples\n19990-1-AP targets Hemoglobin Alpha-Specific in WB, IHC, ELISA applications and shows reactivity with human samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: human placenta tissue, human heart tissue,  human spleen tissue\nPositive IHC detected in: human  colonNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:1000\nImmunohistochemistry (IHC): IHC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nThe hemoglobin molecule is a tetramer consisting of two alpha- and two beta-globin-like chains. HBA1 (hemoglobin alpha chain) protein is a alpha-type chain of hemoglobin encoded by two independent genes (HBA1 and HBA2) whose coding sequences are identical. Two alpha chains coupled with two beta chains constitute the adult hemoglobin (HbA or α2β2). HBA1 is also the component of fetal hemoglobin (HbF or α2γ2). This antibody detects a major band around 14-16 kDa in the western blot analysis of heart tissue and K-562 cells. A higher band around 26-27 kDa can also be observed occasionally, which may represents the dimer form of HBA1 (PMID: 20836851). The antibody is specific to HBA1and has no cross reaction to other globin member.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: Peptide Predict reactive species\nFull Name: hemoglobin, alpha 1\nCalculated Molecular Weight: 16 kDa\nObserved Molecular Weight: 14-16 kDa\nGenBank Accession Number: NM_000558\nGene Symbol: HBA1\nGene ID (NCBI): 3039\nRRID: AB_11182500\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: P69905\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46887666745513,"sku":"19990-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-19990-1-ap","provider":"Iright","version":"1.0","type":"link"}