{"product_id":"proteintech-20139-1-ap","title":"Proteintech, 20139-1-AP, MCT1 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe MCT1 (20139-1-AP) by Proteintech is a Polyclonal antibody targeting MCT1 in WB, IHC, IF\/ICC, IP, ELISA applications with reactivity to human samples\n20139-1-AP targets MCT1 in WB, IHC, IF\/ICC, IP, ELISA applications and shows reactivity with human samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: BxPC-3 cells, HEK-293 cells,  HeLa cells\nPositive IP detected in: Raji cells\nPositive IHC detected in: human colon cancer tissue, human breast cancer tissue,  human cervical cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: HeLa cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:2000-1:10000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:800-1:3200\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nMCT1, the SLC16A1 gene product, is a trans-membrane symporter involved in lactate and pyruvate transportation. It plays an important role in lactic acid transport and H+ clearance in cancer cells. MCT overexpression had been observed in various cancers and may play an important role in tumorigenesis. Two isoforms of MCT1 exist due to the alternative splicing, with predicted MW of 54 kDa and 46 kDa, respectively. While western blot analysis detected MCT1 at an apparent molecular mass of 40-50 kDa.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human\nCited Reactivity: human, mouse, rat, bovine\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag14098 Product name: Recombinant human MCT1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 451-500 aa of BC026317 Sequence: EQKANEQKKESKEEETSIDVAGKPNEVTKAAESPDQKDTDGGPKEEESPV Predict reactive species\nFull Name: solute carrier family 16, member 1 (monocarboxylic acid transporter 1)\nCalculated Molecular Weight: 500 aa, 54 kDa\nObserved Molecular Weight: 38-45 kDa\nGenBank Accession Number: BC026317\nGene Symbol: MCT1\nGene ID (NCBI): 6566\nRRID: AB_2878645\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: P53985\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868828946601,"sku":"20139-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-20139-1-ap","provider":"Iright","version":"1.0","type":"link"}